{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:34:56Z","timestamp":1793198096512,"version":"4.5.0"},"reference-count":60,"publisher":"National Academy of Sciences","issue":"12","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM118833"],"award-info":[{"award-number":["GM118833"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,3,22]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>This study shows that Fragile X mental retardation protein (FMRP) promotes messenger RNA (mRNA)-dependent recombination via facilitating ten-eleven translocation protein 1 (TET1)-mediated mRNA methyl-5-cytosine (m5C) demethylation. Loss of FMRP leads to damage induced mRNA m5C and R-loop accumulation at sites of active transcription, defective recombination repair, and increased radiosensitivity of tumor cells. FMRP-dependent RNA m5C demethylation and R-loop resolving during DNA repair are important for repair completion and the maintenance of genome stability. The removal of m5C by the FMRP\u2013TET1 axis is coupled with R-loop dissolution, which ensures proper completion of DNA repair and survival of cells after DNA damage. These findings significantly advance our understanding of the regulation of RNA modifications in R-loop dynamics during DNA repair.<\/jats:p>","DOI":"10.1073\/pnas.2116251119","type":"journal-article","created":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T14:23:23Z","timestamp":1647354203000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":118,"title":["FMRP promotes transcription-coupled homologous recombination via facilitating TET1-mediated m5C RNA modification demethylation"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3038-6453","authenticated-orcid":false,"given":"Haibo","family":"Yang","sequence":"first","affiliation":[{"name":"Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02129"},{"name":"Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumin","family":"Wang","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02129"},{"name":"Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6732-5649","authenticated-orcid":false,"given":"Yufei","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tribhuwan","family":"Yadav","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02129"},{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02129"},{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laiyee","family":"Phoon","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02129"},{"name":"Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee","family":"Zou","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02129"},{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0383-6172","authenticated-orcid":false,"given":"Li","family":"Lan","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02129"},{"name":"Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2022,3,15]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.01.024"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.04.038"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2549"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3822"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2796"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13682"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06586-3"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1507105112"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11179"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.002"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3643"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.01.048"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","unstructured":"C. Zhang METTL3 and N6- methyladenosine promote homologous recombination-mediated repair of DSBs by modulating DNA-RNA hybrid accumulation. Mol. Cell 79 425\u2013442 (2020).","DOI":"10.1016\/j.molcel.2020.06.017"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16722-7"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b03962"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0361-y"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-020-02417-6"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1478-811X-8-27"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.15698\/cst2019.03.178"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.232"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.01.017"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-05-01539.1997"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00568-2"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.8.080706.092249"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03513-4"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-1139-1"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1233"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90232-1"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2015.02.005"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-018-1122-9"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.040"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00171-0"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.01.029"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.262766.115"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja505305z"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1310656110"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac5253"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad8711"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature21671"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1510"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.55"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.16.00751"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.3109\/10715761003667554"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201302847"},{"key":"e_1_3_4_46_2","first-page":"943","article-title":"Identification of differential expression of genes in hepatocellular carcinoma by suppression subtractive hybridization combined cDNA microarray","volume":"18","author":"Liu Y.","year":"2007","unstructured":"Y. Liu , Identification of differential expression of genes in hepatocellular carcinoma by suppression subtractive hybridization combined cDNA microarray. Oncol. Rep. 18, 943\u2013951 (2007).","journal-title":"Oncol. Rep."},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.521"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu241"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108179"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-18-17"},{"key":"e_1_3_4_51_2","doi-asserted-by":"crossref","unstructured":"A. Chakraborty Fragile X mental retardation protein regulates R-loop formation and prevents global chromosome fragility. bioRxiv [Preprint] (2019). https:\/\/www.biorxiv.org\/content\/10.1101\/601906v2.article-info (Accessed 24 October 2020).","DOI":"10.1101\/601906"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.06.072"},{"key":"e_1_3_4_53_2","first-page":"3936","article-title":"Fragile X mental retardation protein modulates the stability of its m6A-marked messenger RNA targets","volume":"27","author":"Zhang F.","year":"2018","unstructured":"F. Zhang , Fragile X mental retardation protein modulates the stability of its m6A-marked messenger RNA targets. Hum. Mol. Genet. 27, 3936\u20133950 (2018).","journal-title":"Hum. Mol. Genet."},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2020.06.017"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-016-0127-1"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.03.022"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature25434"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.08.055"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-017-9164-1"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.06.033"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2116251119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T07:54:52Z","timestamp":1649836492000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2116251119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,15]]},"references-count":60,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,3,22]]}},"alternative-id":["10.1073\/pnas.2116251119"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2116251119","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,15]]},"assertion":[{"value":"2021-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2116251119"}}