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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,3,29]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Spirochetal pathogens encode an abundance of lipoproteins that can provide a critical interface with the host environment.\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    , the model species for spirochetal biology, must survive an enzootic life cycle defined by fluctuations between vector (tick) and vertebrate host. While\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    expresses over 80 surface lipoproteins\u2014many of which likely contribute to host survival\u2014the\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    lipoproteome is poorly characterized. Here, we generated a platform to rapidly identify targets of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    surface lipoproteins and identified two paralogs that confer resistance to antibody-initiated complement killing that may promote survival in immunocompetent hosts. This work expands our understanding of complement evasion mechanisms and points toward a discovery approach for identifying host\u2013pathogen interactions central to spirochete pathogenesis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2117770119","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T15:15:37Z","timestamp":1647875737000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":27,"title":["Lipoproteome screening of the Lyme disease agent identifies inhibitors of antibody-mediated complement killing"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2613-5862","authenticated-orcid":false,"given":"Michael J.","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts School of Medicine, Tufts University, Boston, MA 02155"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beau","family":"Wager","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts School of Medicine, Tufts University, Boston, MA 02155"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5923-3250","authenticated-orcid":false,"given":"Ryan J.","family":"Garrigues","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Gerlach","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Goethe University Frankfurt, D-60596 Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua D.","family":"Quinn","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts School of Medicine, Tufts University, Boston, MA 02155"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0513-6623","authenticated-orcid":false,"given":"Alexander S.","family":"Dowdell","sequence":"additional","affiliation":[{"name":"Mucosal Inflammation Program, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1072-6828","authenticated-orcid":false,"given":"Marcia S.","family":"Osburne","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts School of Medicine, Tufts University, Boston, MA 02155"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7350-2162","authenticated-orcid":false,"given":"Wolfram R.","family":"Z\u00fcckert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kraiczy","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Goethe University Frankfurt, D-60596 Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6724-8700","authenticated-orcid":false,"given":"Brandon L.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0240-6402","authenticated-orcid":false,"given":"John M.","family":"Leong","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, Tufts School of Medicine, Tufts University, Boston, MA 02155"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2022,3,21]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2702.202731"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.1.411-414.2000"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI19894"},{"key":"e_1_3_4_4_2","first-page":"473","article-title":"Lyme disease pathogenesis","volume":"42","author":"Coburn J.","year":"2021","unstructured":"J. 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