{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:06:06Z","timestamp":1791846366684,"version":"4.3.4"},"reference-count":71,"publisher":"National Academy of Sciences","issue":"28","license":[{"start":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T00:00:00Z","timestamp":1657065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P31470-B30"],"award-info":[{"award-number":["P31470-B30"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P34588-B"],"award-info":[{"award-number":["P34588-B"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,7,12]]},"abstract":"<jats:p>\n                    Abnormal placentation has been noticed in a variety of pregnancy complications such as miscarriage, early-onset preeclampsia, and fetal growth restriction. Defects in the developmental program of extravillous trophoblasts (EVTs), migrating from placental anchoring villi into the maternal decidua and its vessels, is thought to be an underlying cause. Yet, key regulatory mechanisms controlling commitment and differentiation of the invasive trophoblast lineage remain largely elusive. Herein, comparative gene expression analyses of HLA-G\u2013purified EVTs, isolated from donor-matched placenta, decidua, and trophoblast organoids (TB-ORGs), revealed biological processes and signaling pathways governing EVT development. In particular, bioinformatics analyses and manipulations in different versatile trophoblast cell models unraveled transforming growth factor-\u03b2 (TGF-\u03b2) signaling as a crucial pathway driving differentiation of placental EVTs into decidual EVTs, the latter showing enrichment of a secretory gene signature. Removal of Wingless signaling and subsequent activation of the TGF-\u03b2 pathway were required for the formation of human leukocyte antigen-G\n                    <jats:sup>+<\/jats:sup>\n                    (HLA-G\n                    <jats:sup>+<\/jats:sup>\n                    ) EVTs in TB-ORGs that resemble in situ EVTs at the level of global gene expression. Accordingly, TGF-\u03b2\u2013treated EVTs secreted enzymes, such as DAO and PAPPA2, which were predominantly expressed by decidual EVTs. Their genes were controlled by EVT-specific induction and genomic binding of the TGF-\u03b2 downstream effector SMAD3. In summary, TGF-\u03b2 signaling plays a key role in human placental development governing the differentiation program of EVTs.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2120667119","type":"journal-article","created":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T14:25:43Z","timestamp":1657117543000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":105,"title":["Transforming growth factor-\u03b2 signaling governs the differentiation program of extravillous trophoblasts in the developing human placenta"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2409-1317","authenticated-orcid":false,"given":"Sandra","family":"Haider","sequence":"first","affiliation":[{"name":"Placental Development Group, Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Medical University of Vienna, Vienna, 1090 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University of Vienna, Vienna, 1090 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Haslinger","sequence":"additional","affiliation":[{"name":"Fetal-Maternal Immunology Group, Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Medical University of Vienna, Vienna, 1090 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8115-8867","authenticated-orcid":false,"given":"Gudrun","family":"Meinhardt","sequence":"additional","affiliation":[{"name":"Placental Development Group, Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Medical University of Vienna, Vienna, 1090 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Maxian","sequence":"additional","affiliation":[{"name":"Placental Development Group, Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Medical University of Vienna, Vienna, 1090 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Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7457-956X","authenticated-orcid":false,"given":"Paulina A.","family":"Latos","sequence":"additional","affiliation":[{"name":"Center for Anatomy and Cell Biology, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kn\u00f6fler","sequence":"additional","affiliation":[{"name":"Placental Development Group, Reproductive Biology Unit, Department of Obstetrics and Gynaecology, Medical University of Vienna, Vienna, 1090 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Haider Martin Kn\u00f6fler A. Ian Lackner Next generation sequencing of human first trimester placental EVTs (pEVT) decidual interstitial EVTs (iEVT) and trophoblast organoid (TB-ORG)-derived extravillous trophoblasts (TB-ORG-EVTs). GEO Data Base. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE188352. Deposited 6 November 2021."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2120667119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T14:27:25Z","timestamp":1657117645000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.2120667119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,6]]},"references-count":71,"journal-issue":{"issue":"28","published-print":{"date-parts":[[2022,7,12]]}},"alternative-id":["10.1073\/pnas.2120667119"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2120667119","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,6]]},"assertion":[{"value":"2021-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2120667119"}}