{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:35:57Z","timestamp":1781105757459,"version":"3.54.1"},"reference-count":52,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"HHS | NIH | National Cancer Institute","doi-asserted-by":"publisher","award":["R01CA262902"],"award-info":[{"award-number":["R01CA262902"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"HHS | NIH | National Cancer Institute","doi-asserted-by":"publisher","award":["P01CA214091"],"award-info":[{"award-number":["P01CA214091"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,8,9]]},"abstract":"<jats:p>\n                    The germinal center (GC) plays a central role in the generation of antigen-specific B cells and antibodies. Tight regulation of the GC is essential due to the inherent risks of tumorigenesis and autoimmunity posed by inappropriate GC B cell processes. Gammaherpesviruses such as Epstein\u2013Barr virus (EBV) and murine gammaherpesvirus 68 (MHV68) utilize numerous armaments to drive infected na\u00efve B cells, independent of antigen, through GC reactions to expand the latently infected B cell population and establish a stable latency reservoir. We previously demonstrated that the MHV68 microRNA (miRNA)\n                    <jats:italic>mghv-miR-M1-7-5p<\/jats:italic>\n                    represses host\n                    <jats:italic>EWSR1<\/jats:italic>\n                    (Ewing sarcoma breakpoint region 1) to promote B cell infection. EWSR1 is a transcription and splicing regulator that is recognized for its involvement as a fusion protein in Ewing sarcoma. A function for EWSR1 in B cell responses has not been previously reported. Here, we demonstrate that 1) B cell\u2013specific deletion of EWSR1 had no effect on generation of mature B cell subsets or basal immunoglobulin levels in na\u00efve mice, 2) repression or ablation of EWSR1 in B cells promoted expansion of MHV68 latently infected GC B cells, and 3) B cell\u2013specific deletion of EWSR1 during a normal immune response to nonviral antigen resulted in significantly elevated numbers of antigen-specific GC B cells, plasma cells, and circulating antibodies. Notably, EWSR1 deficiency did not affect the proliferation or survival of GC B cells but instead resulted in the generation of increased numbers of precursor GC B cells. Cumulatively, these findings demonstrate that EWSR1 is a negative regulator of B cell responses.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2123362119","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T13:56:05Z","timestamp":1659534965000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":8,"title":["Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3178-3752","authenticated-orcid":false,"given":"Yiping","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"UF Health Cancer Center, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"UF Genetics Institute, College of Medicine, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April","family":"Feswick","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"UF Health Cancer Center, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"UF Genetics Institute, College of Medicine, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5790-1676","authenticated-orcid":false,"given":"Vasiliki","family":"Apostolou","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"UF Health Cancer Center, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"UF Genetics Institute, College of Medicine, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6694-9150","authenticated-orcid":false,"given":"Petko M.","family":"Petkov","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Bar Harbor, ME 04609"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6386-5894","authenticated-orcid":false,"given":"Emily K.","family":"Moser","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"Department of Pathology, College of Medicine, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8889-1642","authenticated-orcid":false,"given":"Scott A.","family":"Tibbetts","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"UF Health Cancer Center, College of Medicine, University of Florida, Gainesville, FL 32610"},{"name":"UF Genetics Institute, College of Medicine, University of Florida, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2022,8,3]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3814"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3804"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-120419-022408"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.03.016"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1589"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204640"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2637"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra032015"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2888"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens5010018"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-072710-081639"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-011921-082615"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.8.5894-5904.1997"},{"key":"e_1_3_4_14_2","first-page":"818","article-title":"Lymphoproliferative disease in mice infected with murine gammaherpesvirus 68","volume":"145","author":"Sunil-Chandra N. 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