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Structurally, sexual dysfunction was associated with a substantial loss of neuronal nitric oxide synthase (nNOS)-expressing neurons in the ventromedial nucleus of the hypothalamus (VMH) and other areas of hypothalamic nuclei involved in social behaviors. Using in\u00a0vivo chemogenetic manipulation, we show that nNOS\n                    <jats:sup>VMH<\/jats:sup>\n                    neurons are required for the display of normal sexual behavior in female mice and that pharmacological replenishment of nitric oxide restores normal sexual performance in PAMH mice. Our data provide a framework to investigate facets of hypothalamic nNOS neuron biology with implications for sexual disturbances in PCOS.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2203503119","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T14:08:49Z","timestamp":1658498929000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":26,"title":["Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4447-6388","authenticated-orcid":false,"given":"Mauro S. 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