{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T09:28:31Z","timestamp":1791365311540,"version":"4.3.1"},"reference-count":62,"publisher":"National Academy of Sciences","issue":"41","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS096281"],"award-info":[{"award-number":["NS096281"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS121103"],"award-info":[{"award-number":["NS121103"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS112541"],"award-info":[{"award-number":["NS112541"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,10,10]]},"abstract":"<jats:p>\n                    Neurobiological consequences of traumatic brain injury (TBI) result from a complex interplay of secondary injury responses and sequela that mediates chronic disability. Endothelial cells are important regulators of the cerebrovascular response to TBI. Our work demonstrates that genetic deletion of endothelial cell (EC)-specific EPH receptor A4 (EphA4) using conditional\n                    <jats:italic>\n                      EphA4\n                      <jats:sup>f\/f<\/jats:sup>\n                      \/Tie2-Cre\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      EphA4\n                      <jats:sup>f\/f<\/jats:sup>\n                      \/VE-Cadherin-CreERT2\n                    <\/jats:italic>\n                    knockout (KO) mice promotes blood\u2013brain barrier (BBB) integrity and tissue protection, which correlates with improved motor function and cerebral blood flow recovery following controlled cortical impact (CCI) injury. scRNAseq of capillary-derived KO ECs showed increased differential gene expression of BBB-related junctional and actin cytoskeletal regulators, namely, A-kinase anchor protein 12,\n                    <jats:italic>Akap12<\/jats:italic>\n                    , whose presence at Tie2 clustering domains is enhanced in KO microvessels. Transcript and protein analysis of CCI-injured whole cortical tissue or cortical-derived ECs suggests that EphA4 limits the expression of Cldn5, Akt, and Akap12 and promotes Ang2. Blocking Tie2 using sTie2-Fc attenuated protection and reversed Akap12 mRNA and protein levels cortical-derived ECs. Direct stimulation of Tie2 using Vasculotide, angiopoietin-1 memetic peptide, phenocopied the neuroprotection. Finally, we report a noteworthy rise in soluble Ang2 in the sera of individuals with acute TBI, highlighting its promising role as a vascular biomarker for early detection of BBB disruption. These findings describe a contribution of the axon guidance molecule, EphA4, in mediating TBI microvascular dysfunction through negative regulation of Tie2\/Akap12 signaling.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2204700120","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T13:58:08Z","timestamp":1696514288000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":27,"title":["Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2\/Akap12 signaling to preserve blood\u2013brain barrier integrity"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6807-9366","authenticated-orcid":false,"given":"Alison","family":"Cash","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, VA 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