{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T12:07:29Z","timestamp":1793189249953,"version":"4.5.0"},"reference-count":79,"publisher":"National Academy of Sciences","issue":"48","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"HHS | NIH | National Eye Institute","doi-asserted-by":"publisher","award":["EY023295"],"award-info":[{"award-number":["EY023295"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"HHS | NIH | National Eye Institute","doi-asserted-by":"publisher","award":["EY024932"],"award-info":[{"award-number":["EY024932"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"HHS | NIH | National Eye Institute","doi-asserted-by":"publisher","award":["EY028106"],"award-info":[{"award-number":["EY028106"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"HHS | NIH | National Eye Institute","doi-asserted-by":"publisher","award":["EY032518"],"award-info":[{"award-number":["EY032518"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"HHS | NIH | National Eye Institute","doi-asserted-by":"publisher","award":["EY026877"],"award-info":[{"award-number":["EY026877"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001641","name":"Glaucoma Research Foundation","doi-asserted-by":"publisher","award":["CFC3"],"award-info":[{"award-number":["CFC3"]}],"id":[{"id":"10.13039\/100001641","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006312","name":"BrightFocus Foundation","doi-asserted-by":"publisher","award":["G2018183"],"award-info":[{"award-number":["G2018183"]}],"id":[{"id":"10.13039\/100006312","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["NDCN"],"award-info":[{"award-number":["NDCN"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,11,29]]},"abstract":"<jats:p>\n                    Retinal ganglion cells (RGCs) are heterogeneous projection neurons that convey distinct visual features from the retina to brain. Here, we present a high-throughput in vivo RGC activity assay in response to light stimulation using noninvasive Ca\n                    <jats:sup>2+<\/jats:sup>\n                    imaging of thousands of RGCs simultaneously in living mice. Population and single-cell analyses of longitudinal RGC Ca\n                    <jats:sup>2+<\/jats:sup>\n                    imaging reveal distinct functional responses of RGCs and unprecedented individual RGC activity conversions during traumatic and glaucomatous degeneration. This study establishes a foundation for future in vivo RGC function classifications and longitudinal activity evaluations using more advanced imaging techniques and visual stimuli under normal, disease, and neural repair conditions. These analyses can be performed at both the population and single-cell levels using temporal and spatial information, which will be invaluable for understanding RGC pathophysiology and identifying functional biomarkers for diverse optic neuropathies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2206829119","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T15:18:11Z","timestamp":1669043891000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["Longitudinal in vivo Ca\n                    <sup>2+<\/sup>\n                    imaging reveals dynamic activity changes of diseased retinal ganglion cells at the single-cell level"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6292-5995","authenticated-orcid":false,"given":"Liang","family":"Li","sequence":"first","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9624-9665","authenticated-orcid":false,"given":"Xue","family":"Feng","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Fang","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"},{"name":"Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha 410011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1630-1233","authenticated-orcid":false,"given":"David A.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaobo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of California San Francisco, San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoliang","family":"Huang","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1601-2818","authenticated-orcid":false,"given":"Pingting","family":"Liu","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2099-0190","authenticated-orcid":false,"given":"Junting","family":"Liu","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nripun","family":"Sredar","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4712-7626","authenticated-orcid":false,"given":"Liang","family":"Liu","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Sun","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of California San Francisco, San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey L.","family":"Goldberg","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5089-1196","authenticated-orcid":false,"given":"Hao F.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7980-1649","authenticated-orcid":false,"given":"Yang","family":"Hu","sequence":"additional","affiliation":[{"name":"Spencer Center for Vision Research, Department of Ophthalmology, Byers Eye Institute at Stanford University School of Medicine, Palo Alto, CA 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2022,11,21]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-071714-034120"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2018.06.003"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00055735-199712000-00003"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2010.09.005"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/ICU.0000000000000473"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddx273"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2014.05.013"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","unstructured":"D. J. Calkins Adaptive responses to neurodegenerative stress in glaucoma. Prog. Retin. Eye Res. 84 100953 (2021) 10.1016\/j.preteyeres.2021.100953 100953.","DOI":"10.1016\/j.preteyeres.2021.100953"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-10428"},{"key":"e_1_3_4_10_2","first-page":"2654","article-title":"Properties of perimetric threshold estimates from full threshold, SITA standard, and SITA fast strategies","volume":"43","author":"Artes P. H.","year":"2002","unstructured":"P. H. Artes, A. Iwase, Y. Ohno, Y. Kitazawa, B. C. Chauban, Properties of perimetric threshold estimates from full threshold, SITA standard, and SITA fast strategies. Invest. Ophth. Vis. Sci. 43, 2654\u20132659 (2002).","journal-title":"Invest. Ophth. Vis. Sci."},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2007.08.001"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-2697"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0135"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2014.01.020"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2016.03.006"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.05.008"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.14-13943"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-10720"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-11188"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00109"},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","unstructured":"J. Zhang Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse. eLife 8 e45881 (2019). e45881","DOI":"10.7554\/eLife.45881"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2020.108117"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.3791\/60409"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-88690-x"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.11.006"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-07-02737.2002"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2012.08.017"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09424"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature22818"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2778-13.2013"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01980-9"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-14-06063.2003"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180091"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2804-16.2016"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4359"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1398"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2130-2"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.07.011"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature16468"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00852.2012"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040352"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-6012"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2014.10.003"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01043.2012"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0194947"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0435-6"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6416"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.14908"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-1289-z"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0102-20.2020"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0629-11.2011"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/353655a0"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200609490"},{"key":"e_1_3_4_54_2","doi-asserted-by":"crossref","unstructured":"F. M. Nadal-Nicolas True S-cones are concentrated in the ventral mouse retina and wired for color detection in the upper visual field. eLife 9 e56840 (2020). e56840","DOI":"10.7554\/eLife.56840"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.09.030"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1198\/106186006X113430"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v084.i10"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1198\/016214502760047131"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00389-1"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00481"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019330"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2012.07.001"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-009-1155-x"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1097\/ICU.0000000000000238"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2021.108571"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1419-14.2015"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0940-16.2016"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00219"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030677"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2104884118"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aas9160"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-14-06063.2003"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4438-13.2014"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41377-020-0317-9"},{"key":"e_1_3_4_75_2","doi-asserted-by":"crossref","unstructured":"T. Kamali S. R. M. Farrell W. H. Baldridge J. Fischer B. C. Chauhan \u201cTwo-photon scanning laser ophthalmoscope\u201d in High Resolution Imaging in Microscopy and Ophthalmology: New Frontiers in Biomedical Optics J. F. Bille Ed. (Springer Cham (CH) 2019) pp. 195\u2013211 10.1007\/978-3-030-16638-0_9.","DOI":"10.1007\/978-3-030-16638-0_9"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.4.001285"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/lsa.2016.7"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.11.004"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-0762-7"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2206829119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T08:24:48Z","timestamp":1695284688000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2206829119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,21]]},"references-count":79,"journal-issue":{"issue":"48","published-print":{"date-parts":[[2022,11,29]]}},"alternative-id":["10.1073\/pnas.2206829119"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2206829119","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,21]]},"assertion":[{"value":"2022-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2206829119"}}