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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,2,21]]},"abstract":"<jats:p>\n                    Sperm acrosomal membrane proteins, such as Izumo sperm\u2013egg fusion 1 (IZUMO1) and sperm acrosome-associated 6 (SPACA6), play essential roles in mammalian gamete binding or fusion. How their biosynthesis is regulated during spermiogenesis has largely remained elusive. Here, we show that\n                    <jats:italic>1700029I15Rik<\/jats:italic>\n                    knockout male mice are severely subfertile and their spermatozoa do not fuse with eggs. 1700029I15Rik is a type-II transmembrane protein expressed in early round spermatids but not in mature spermatozoa. It interacts with proteins involved in\n                    <jats:italic>N<\/jats:italic>\n                    -linked glycosylation, disulfide isomerization, and endoplasmic reticulum (ER)\u2013Golgi trafficking, suggesting a potential role in nascent protein processing. The ablation of\n                    <jats:italic>1700029I15Rik<\/jats:italic>\n                    destabilizes non-catalytic subunits of the oligosaccharyltransferase (OST) complex that are pivotal for\n                    <jats:italic>N<\/jats:italic>\n                    -glycosylation. The knockout testes exhibit normal expression of sperm plasma membrane proteins, but decreased abundance of multiple acrosomal membrane proteins involved in fertilization. The knockout sperm show upregulated chaperones related to ER-associated degradation (ERAD) and elevated protein ubiquitination; strikingly, SPACA6 becomes undetectable. Our results support for a specific, 1700029I15Rik-mediated pathway underpinning the biosynthesis of acrosomal membrane proteins during spermiogenesis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2207263120","type":"journal-article","created":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T13:54:36Z","timestamp":1676382876000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm\u2013egg interaction"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0198-8906","authenticated-orcid":false,"given":"Yonggang","family":"Lu","sequence":"first","affiliation":[{"name":"Immunology Frontier Research Center, Osaka University, Osaka 565-0871, Japan"},{"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Shimada","sequence":"additional","affiliation":[{"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan"},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3904-492X","authenticated-orcid":false,"given":"Shaogeng","family":"Tang","sequence":"additional","affiliation":[{"name":"Sarafan ChEM-H, Stanford University, Stanford, CA 94305"},{"name":"Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-41390, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yo","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan"},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0260-7861","authenticated-orcid":false,"given":"Taichi","family":"Noda","sequence":"additional","affiliation":[{"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan"},{"name":"Division of Reproductive Biology, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, Japan"},{"name":"Priority Organization for Innovation and Excellence, Kumamoto University, Kumamoto 860-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3400-0741","authenticated-orcid":false,"given":"Hiroki","family":"Shibuya","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-41390, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9859-6217","authenticated-orcid":false,"given":"Masahito","family":"Ikawa","sequence":"additional","affiliation":[{"name":"Immunology Frontier Research Center, Osaka University, Osaka 565-0871, Japan"},{"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan"},{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan"},{"name":"Laboratory of Reproductive Systems Biology, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"},{"name":"Center for Infectious Disease Education and Research, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,2,14]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI41585"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.260296"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03362"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.810118"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-013-9491-x"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1922650117"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62091-y"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1917060117"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","unstructured":"I. 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Roboti, S. High, The oligosaccharyltransferase subunits OST48, DAD1 and KCP2 function as ubiquitous and selective modulators of mammalian N-glycosylation. J. Cell Sci. 125, 3474\u20133484 (2012).","journal-title":"J. Cell Sci."},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201702159"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.394288"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)90843-A"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1063"},{"key":"e_1_3_4_42_2","doi-asserted-by":"crossref","unstructured":"W. Contreras C11orf94\/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly. Sci. 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