{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:19:41Z","timestamp":1793405981229,"version":"4.5.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"34","license":[{"start":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T00:00:00Z","timestamp":1660521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,8,23]]},"abstract":"<jats:p>\n                    The targeted delivery of messenger RNA (mRNA) to desired organs remains a great challenge for in\u00a0vivo applications of mRNA technology. For mRNA vaccines, the targeted delivery to the lymph node (LN) is predicted to reduce side effects and increase the immune response. In this study, we explored an endogenously LN-targeting lipid nanoparticle (LNP) without the modification of any active targeting ligands for developing an mRNA cancer vaccine. The LNP named 113-O12B showed increased and specific expression in the LN compared with LNP formulated with ALC-0315, a synthetic lipid used in the COVID-19 vaccine Comirnaty. The targeted delivery of mRNA to the LN increased the CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell response to the encoded full-length ovalbumin (OVA) model antigen. As a result, the protective and therapeutic effect of the OVA-encoding mRNA vaccine on the OVA-antigen\u2013bearing B16F10 melanoma model was also improved. Moreover, 113-O12B encapsulated with TRP-2 peptide (TRP2\n                    <jats:sub>180\u2013188<\/jats:sub>\n                    )\u2013encoding mRNA also exhibited excellent tumor inhibition, with the complete response of 40% in the regular B16F10 tumor model when combined with anti\u2013programmed death-1 (PD-1) therapy, revealing broad application of 113-O12B from protein to peptide antigens. All the treated mice showed long-term immune memory, hindering the occurrence of tumor metastatic nodules in the lung in the rechallenging experiments that followed. The enhanced antitumor efficacy of the LN-targeting LNP system shows great potential as a universal platform for the next generation of mRNA vaccines.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2207841119","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T15:21:26Z","timestamp":1660576886000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":405,"title":["Lipid nanoparticle-mediated lymph node\u2013targeting delivery of mRNA cancer vaccine elicits robust CD8\n                    <sup>+<\/sup>\n                    T cell response"],"prefix":"10.1073","volume":"119","author":[{"given":"Jinjin","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, MA 02155"},{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong\u2013Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0383-7247","authenticated-orcid":false,"given":"Zhongfeng","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, MA 02155"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changfeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, MA 02155"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Tufts University, Medford, MA 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