{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:09:21Z","timestamp":1790834961015,"version":"4.1.0"},"reference-count":67,"publisher":"National Academy of Sciences","issue":"48","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017540","name":"HHS | NIH | NHLBI | NHLBI Division of Intramural Research","doi-asserted-by":"publisher","award":["1ZIAHD008893-07"],"award-info":[{"award-number":["1ZIAHD008893-07"]}],"id":[{"id":"10.13039\/100017540","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,11,29]]},"abstract":"<jats:p>\n                    Intravacuolar pathogens need to gradually expand their surrounding vacuole to accommodate the growing number of bacterial offspring during intracellular replication. Here we found that\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    controls vacuole expansion by fine-tuning the generation of lysophospholipids within the vacuolar membrane. Upon allosteric activation by binding to host ubiquitin, the type IVB (Dot\/Icm) effector VpdC converts phospholipids into lysophospholipids which, at moderate concentrations, are known to promote membrane fusion but block it at elevated levels by generating excessive positive membrane curvature. Consequently,\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    overproducing VpdC were prevented from adequately expanding their surrounding membrane, trapping the replicating bacteria within spatially confined vacuoles and reducing their capability to proliferate intracellularly. Quantitative lipidomics confirmed a VpdC-dependent increase in several types of lysophospholipids during infection, and VpdC production in transiently transfected cells caused tubulation of organelle membranes as well as mitochondria fragmentation, processes that can be phenocopied by supplying cells with exogenous lysophospholipids. Together, these results demonstrate an important role for bacterial phospholipases in vacuolar expansion.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2209149119","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T13:10:35Z","timestamp":1669122635000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["VpdC is a ubiquitin-activated phospholipase effector that regulates\n                    <i>Legionella<\/i>\n                    vacuole expansion during infection"],"prefix":"10.1073","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4187-1430","authenticated-orcid":false,"given":"Xiao","family":"Li","sequence":"first","affiliation":[{"name":"Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6838-0201","authenticated-orcid":false,"given":"D. Eric","family":"Anderson","sequence":"additional","affiliation":[{"name":"Advanced Mass Spectrometry Core, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4952-8941","authenticated-orcid":false,"given":"Yuen-Yan","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1269-3508","authenticated-orcid":false,"given":"Michal","family":"Jarnik","sequence":"additional","affiliation":[{"name":"Neurosciences and Cellular and Structural Biology Division, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6971-7451","authenticated-orcid":false,"given":"Matthias P.","family":"Machner","sequence":"additional","affiliation":[{"name":"Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2022,11,21]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1874-5245(97)80006-7"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-38918-7_90"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00173a027"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1455"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.11.4231-4237.1995"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.8.3941-3951.1998"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10010027"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2017.10.002"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002988"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/019075-0"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2008.08.012"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/379372"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302472200"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1405391111"},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","unstructured":"B. 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