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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,10,25]]},"abstract":"<jats:p>\n                    Mucins are large, highly glycosylated transmembrane and secreted proteins that line and protect epithelial surfaces. However, the details of mucin biosynthesis and packaging in\u00a0vivo are largely unknown. Here, we demonstrate that multiple distinct mucins undergo intragranular restructuring during secretory granule maturation in\u00a0vivo, forming unique structures that are spatially segregated within the same granule. We further identify temporally-regulated genes that influence mucin restructuring, including those controlling pH (\n                    <jats:italic>Vha16-1<\/jats:italic>\n                    ), Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ions (\n                    <jats:italic>fwe<\/jats:italic>\n                    ) and Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    ions (\n                    <jats:italic>Clic<\/jats:italic>\n                    and\n                    <jats:italic>ClC-c<\/jats:italic>\n                    ). Finally, we show that altered mucin glycosylation influences the dimensions of these structures, thereby affecting secretory granule morphology. This study elucidates key steps and factors involved in intragranular, rather than intergranular segregation of mucins through regulated restructuring events during secretory granule maturation. Understanding how multiple distinct mucins are efficiently packaged into and secreted from secretory granules may provide insight into diseases resulting from defects in mucin secretion.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2209750119","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T15:10:57Z","timestamp":1666019457000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":26,"title":["Regulated Restructuring of Mucins During Secretory Granule Maturation In\u00a0Vivo"],"prefix":"10.1073","volume":"119","author":[{"given":"Zulfeqhar A.","family":"Syed","sequence":"first","affiliation":[{"name":"Developmental Glycobiology Section, NIDCR, National Institutes of Health, Bethesda, MD 20892-4370"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8637-4975","authenticated-orcid":false,"given":"Liping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Developmental Glycobiology Section, NIDCR, National Institutes of Health, Bethesda, MD 20892-4370"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duy T.","family":"Tran","sequence":"additional","affiliation":[{"name":"NIDCR Imaging Core, NIDCR, National Institutes of Health, Bethesda, MD 20892-4370"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1029-3444","authenticated-orcid":false,"given":"Christopher K. 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