{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:33:42Z","timestamp":1781109222464,"version":"3.54.1"},"reference-count":70,"publisher":"National Academy of Sciences","issue":"45","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000082","name":"NSF | EHR | Division of Graduate Education","doi-asserted-by":"publisher","award":["DGE-1650112"],"award-info":[{"award-number":["DGE-1650112"]}],"id":[{"id":"10.13039\/100000082","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000078","name":"NSF | MPS | Division of Materials Research","doi-asserted-by":"publisher","award":["DMR-2011924"],"award-info":[{"award-number":["DMR-2011924"]}],"id":[{"id":"10.13039\/100000078","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM118290"],"award-info":[{"award-number":["R35GM118290"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2022,11,8]]},"abstract":"<jats:p>\n                    UV radiation (UVR) has significant physiological effects on organisms living at or near the Earth\u2019s surface, yet the full suite of genes required for fitness of a photosynthetic organism in a UVR-rich environment remains unknown. This study reports a genome-wide fitness assessment of the genes that affect UVR tolerance under environmentally relevant UVR dosages in the model cyanobacterium\n                    <jats:italic>Synechococcus elongatus<\/jats:italic>\n                    PCC 7942. Our results highlight the importance of specific genes that encode proteins involved in DNA repair, glutathione synthesis, and the assembly and maintenance of photosystem II, as well as genes that encode hypothetical proteins and others without an obvious connection to canonical methods of UVR tolerance. Disruption of a gene that encodes a leucyl aminopeptidase (LAP) conferred the greatest UVR-specific decrease in fitness. Enzymatic assays demonstrated a strong pH-dependent affinity of the LAP for the dipeptide cysteinyl-glycine, suggesting an involvement in glutathione catabolism as a function of night-time cytosolic pH level. A low differential expression of the LAP gene under acute UVR exposure suggests that its relative importance would be overlooked in transcript-dependent screens. Subsequent experiments revealed a similar UVR-sensitivity phenotype in LAP knockouts of other organisms, indicating conservation of the functional role of LAPs in UVR tolerance.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2211789119","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T14:50:06Z","timestamp":1667400606000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":7,"title":["An unexpected role for leucyl aminopeptidase in UV tolerance revealed by a genome-wide fitness assessment in a model cyanobacterium"],"prefix":"10.1073","volume":"119","author":[{"given":"Elliot L.","family":"Weiss","sequence":"first","affiliation":[{"name":"Integrative Oceanography Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093"},{"name":"Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxu","family":"Fang","sequence":"additional","affiliation":[{"name":"Center for Circadian Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Taton","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Szubin","sequence":"additional","affiliation":[{"name":"Bioengineering Department, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2357-6785","authenticated-orcid":false,"given":"Bernhard \u00d8.","family":"Palsson","sequence":"additional","affiliation":[{"name":"Bioengineering Department, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. Greg","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Integrative Oceanography Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4264-7019","authenticated-orcid":false,"given":"Susan S.","family":"Golden","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of California San Diego, La Jolla, CA 92093"},{"name":"Center for Circadian Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"12","article-title":"Transmission of ultraviolet light in the Weddell Sea: Report of the first measurements made in the Antarctic","volume":"12","author":"Gieskes W. W. C.","year":"1990","unstructured":"W. W. C. Gieskes, G. W. Kraay, Transmission of ultraviolet light in the Weddell Sea: Report of the first measurements made in the Antarctic. 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Simkovsky Transcriptomic and phenomic investigations reveal elements in biofilm repression and formation in the cyanobacterium Synechococcus elongatus PCC 7942. Front. Microbiol.\u00a0 13 899150 (2022).","DOI":"10.3389\/fmicb.2022.899150"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-007-9170-4"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.33"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605709103"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-96432-2"},{"key":"e_1_3_4_17_2","first-page":"155","volume-title":"Methods Mol. Biol.","author":"Clerico E. M.","unstructured":"E. M. Clerico, J. L. Ditty, S. S. Golden, Specialized techniques for site-directed mutagenesis in cyanobacteria. Methods Mol. 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Chen, C. K. Holtman, A. Taton, S. S. Golden, Functional analysis of the Synechococcus elongatus PCC 7942 genome: Functional genomics and evolution of photosynthetic systems. Advances in Photosynthesis and Respiration, eds Burnap R, Vermaas W (Springer, The Netherlands), 33, 119\u2013137 (2012)."},{"key":"e_1_3_4_70_2","unstructured":"\u00a0University\u00a0of\u00a0California\u00a0San\u00a0Diego.\u00a0An unexpected role for leucyl aminopeptidase in UV tolerance revealed by a genome-wide fitness assessment in a model cyanobacterium. NCBI BioProject.\u00a0http:\/\/www.ncbi.nlm.nih.gov\/sra\/?term=PRJNA854269.\u00a0Deposited\u00a029\u00a0June\u00a02022."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2211789119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T08:31:16Z","timestamp":1695285076000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2211789119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,2]]},"references-count":70,"journal-issue":{"issue":"45","published-print":{"date-parts":[[2022,11,8]]}},"alternative-id":["10.1073\/pnas.2211789119"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2211789119","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,2]]},"assertion":[{"value":"2022-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2211789119"}}