{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T21:28:51Z","timestamp":1789334931235,"version":"build-2803163510"},"reference-count":52,"publisher":"National Academy of Sciences","issue":"28","license":[{"start":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K06638"],"award-info":[{"award-number":["20K06638"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15J04920"],"award-info":[{"award-number":["15J04920"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018732","name":"Ohsumi Frontier Science Foundation","doi-asserted-by":"publisher","award":["Research Grant"],"award-info":[{"award-number":["Research Grant"]}],"id":[{"id":"10.13039\/501100018732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"UKRI | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["GEN BB\/P013511\/1"],"award-info":[{"award-number":["GEN BB\/P013511\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019238","name":"\u6771\u4eac\u5de5\u696d\u5927\u5b66 | Institute of Innovative Research, Tokyo Institute of Technology","doi-asserted-by":"publisher","award":["WRHI program"],"award-info":[{"award-number":["WRHI program"]}],"id":[{"id":"10.13039\/501100019238","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,7,11]]},"abstract":"<jats:p>\n                    The malaria parasite\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    has a nonphotosynthetic plastid called the apicoplast, which contains its own genome. Regulatory mechanisms for apicoplast gene expression remain poorly understood, despite this organelle being crucial for the parasite life cycle. Here, we identify a nuclear-encoded apicoplast RNA polymerase \u03c3 subunit (sigma factor) which, along with the \u03b1 subunit, appears to mediate apicoplast transcript accumulation. This has a periodicity reminiscent of parasite circadian or developmental control. Expression of the apicoplast subunit gene,\n                    <jats:italic>apSig<\/jats:italic>\n                    , together with apicoplast transcripts, increased in the presence of the blood circadian signaling hormone melatonin. Our data suggest that the host circadian rhythm is integrated with intrinsic parasite cues to coordinate apicoplast genome transcription. This evolutionarily conserved regulatory system might be a future target for malaria treatment.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2214765120","type":"journal-article","created":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T13:48:05Z","timestamp":1688564885000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":9,"title":["Coordination of apicoplast transcription in a malaria parasite by internal and host cues"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6200-964X","authenticated-orcid":false,"given":"Yuki","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6077-3000","authenticated-orcid":false,"given":"Keisuke","family":"Komatsuya","sequence":"additional","affiliation":[{"name":"Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan"},{"name":"Laboratory of Biomembrane, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3040-4757","authenticated-orcid":false,"given":"Sousuke","family":"Imamura","sequence":"additional","affiliation":[{"name":"Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan"},{"name":"Space Environment and Energy Laboratories, Nippon Telegraph and Telephone Corporation, Tokyo 180-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyoshi","family":"Nozaki","sequence":"additional","affiliation":[{"name":"Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoh-ichi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2738-430X","authenticated-orcid":false,"given":"Shigeharu","family":"Sato","sequence":"additional","affiliation":[{"name":"Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan"},{"name":"Department of Pathology and Microbiology, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Sabah 88400, Malaysia"},{"name":"Borneo Medical and Health Research Centre, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Sabah 88400, Malaysia"},{"name":"School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6859-0105","authenticated-orcid":false,"given":"Antony N.","family":"Dodd","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7RU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0426-4308","authenticated-orcid":false,"given":"Kiyoshi","family":"Kita","sequence":"additional","affiliation":[{"name":"Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan"},{"name":"School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki 852-8523, Japan"},{"name":"Department of Host-Defense Biochemistry, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7560-7884","authenticated-orcid":false,"given":"Kan","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,7,5]]},"reference":[{"key":"e_1_3_5_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/35017112"},{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba2658"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-12-372"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2010.2457"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-010-0250-5"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2018.05.010"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001138"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.06.030"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04966-3"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0000005"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.68.2215"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2010.06.016"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1230397"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00223-7"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2016.07.004"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2008.03.007"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom5031245"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt302"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.06.081"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.16.4628-4631.2000"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.12.3843-3849.1992"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2017.1356568"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba4357"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw405"},{"key":"e_1_3_5_25_2","first-page":"3","article-title":"The human circadian system in normal and disordered sleep","volume":"66","author":"Richardson G. S.","year":"2005","unstructured":"G. S. Richardson, The human circadian system in normal and disordered sleep. J. Clin. Psychiatry 66, 3\u20139 (2005).","journal-title":"J. Clin. Psychiatry"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.02.070"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm5010978"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004520"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.864819"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02588.x"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04025.x"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003655"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad9279"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2017.12.005"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1177\/074873001129002114"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-021-03643-z"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1994013235"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.781840"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.2307\/3280287"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(80)90003-X"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1821-1827.1999"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.07.019"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2006.00229.x"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04065-7"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2203"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.03.071"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042977"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13859"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1177\/0748730410379711"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti789"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2214765120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T09:32:00Z","timestamp":1695288720000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2214765120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,5]]},"references-count":52,"journal-issue":{"issue":"28","published-print":{"date-parts":[[2023,7,11]]}},"alternative-id":["10.1073\/pnas.2214765120"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2214765120","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,5]]},"assertion":[{"value":"2022-08-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2214765120"}}