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In this regard, we previously showed that\n                    <jats:italic>Mycoplasma fermentans<\/jats:italic>\n                    DnaK, an HSP70 chaperone protein, hampers the activity of important cellular proteins responsible for DNA integrity. Here, we describe a novel DnaK knock-in mouse model generated in our laboratory to study the effect of\n                    <jats:italic>M. fermentans<\/jats:italic>\n                    DnaK expression in vivo. By using an array-based comparative genomic hybridization assay, we demonstrate that exposure to DnaK was associated with a higher number of DNA copy number variants (CNVs) indicative of unbalanced chromosomal alterations, together with reduced fertility and a high rate of fetal abnormalities. Consistent with their implication in genetic disorders, one of these CNVs caused a homozygous\n                    <jats:italic>Grid2<\/jats:italic>\n                    deletion, resulting in an aberrant ataxic phenotype that recapitulates the extensive biallelic deletion in the\n                    <jats:italic>Grid2<\/jats:italic>\n                    gene classified in humans as autosomal recessive spinocerebellar ataxia 18. Our data highlight a connection between components of the human urogenital tract microbiota, namely\n                    <jats:italic>Mycoplasmas<\/jats:italic>\n                    , and genetic abnormalities in the form of DNA CNVs, with obvious relevant medical, diagnostic, and therapeutic implications.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2219897120","type":"journal-article","created":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T15:12:34Z","timestamp":1689606754000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":8,"title":["Mycoplasma DnaK increases DNA copy number variants in vivo"],"prefix":"10.1073","volume":"120","author":[{"given":"Francesca","family":"Benedetti","sequence":"first","affiliation":[{"name":"Institute of Human Virology and Global Virus Network Center, University of Maryland School of Medicine, Baltimore, MD 21201"},{"name":"Department of Biochemistry and Molecular Biology, University 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