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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,5,2]]},"abstract":"<jats:p>\n                    SNIO\u2013CBP, a single-nanometer iron oxide (SNIO) nanoparticle functionalized with a type I collagen-binding peptide (CBP), was developed as a\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    -weighted MRI contrast agent with only endogenous elements for fast and noninvasive detection of liver fibrosis. SNIO\u2013CBP exhibits 6.7-fold higher relaxivity compared to a molecular gadolinium-based collagen-binding contrast agent CM-101 on a per CBP basis at 4.7\u00a0T. Unlike most iron oxide nanoparticles, SNIO\u2013CBP exhibits fast elimination from the bloodstream with a 5.7\u00a0min half-life, high renal clearance, and low, transient liver enhancement in healthy mice. We show that a dose of SNIO\u2013CBP that is 2.5-fold lower than that for CM-101 has comparable imaging efficacy in rapid (within 15\u00a0min following intravenous injection) detection of hepatotoxin-induced liver fibrosis using\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    -weighted MRI in a carbon tetrachloride\u2013induced mouse liver injury model. We further demonstrate the applicability of SNIO\u2013CBP in detecting liver fibrosis in choline-deficient\n                    <jats:italic>L<\/jats:italic>\n                    -amino acid-defined high-fat diet mouse model of nonalcoholic steatohepatitis. These results provide a platform with potential for the development of high relaxivity, gadolinium-free molecular MRI probes for characterizing chronic liver disease.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2220036120","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T15:12:52Z","timestamp":1682349172000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":30,"title":["Fast detection of liver fibrosis with collagen-binding single-nanometer iron oxide nanoparticles via\n                    <i>T<\/i>\n                    <sub>1<\/sub>\n                    -weighted MRI"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1827-3395","authenticated-orcid":false,"given":"Juanye","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Ning","sequence":"additional","affiliation":[{"name":"Athinoula A. 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