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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,2,28]]},"abstract":"<jats:p>\n                    The activation of thymic B cells is critical for their licensing as antigen presenting cells and resulting ability to mediate T cell central tolerance. The processes leading to licensing are still not fully understood. By comparing thymic B cells to activated Peyer\u2019s patch B cells at steady state, we found that thymic B cell activation starts during the neonatal period and is characterized by TCR\/CD40-dependent activation, followed by immunoglobulin class switch recombination (CSR) without forming germinal centers. Transcriptional analysis also demonstrated a strong interferon signature, which was not apparent in the periphery. Thymic B cell activation and CSR were primarily dependent on type III IFN signaling, and loss of type III IFN receptor in thymic B cells resulted in reduced thymocyte regulatory T cell (T\n                    <jats:sub>reg<\/jats:sub>\n                    ) development. Finally, from TCR deep sequencing, we estimate that licensed B cells induce development of a substantial fraction of the T\n                    <jats:sub>reg<\/jats:sub>\n                    cell repertoire. Together, these findings reveal the importance of steady-state type III IFN in generating licensed thymic B cells that induce T cell tolerance to activated B cells.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2220120120","type":"journal-article","created":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T13:58:31Z","timestamp":1676987911000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":46,"title":["Type III interferon drives thymic B cell activation and regulatory T cell generation"],"prefix":"10.1073","volume":"120","author":[{"given":"Ryan J.","family":"Martinez","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise R.","family":"Breed","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosan","family":"Worota","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine M.","family":"Ashby","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3672-7629","authenticated-orcid":false,"given":"Matou\u0161","family":"Vobo\u0159il","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tailor","family":"Mathes","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar C.","family":"Salgado","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine H.","family":"O\u2019Connor","sequence":"additional","affiliation":[{"name":"Research Informatics Solutions, Laboratory Medicine and Pathology Group, Minnesota Supercomputing Institute, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei V.","family":"Kotenko","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Molecular Genetics, Rutgers New Jersey Medical School, Newark, NJ 07103"},{"name":"Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ 07103"},{"name":"Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, NJ 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin A.","family":"Hogquist","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,2,21]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1707"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","unstructured":"E. 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