{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:19:42Z","timestamp":1791350382512,"version":"4.3.1"},"reference-count":51,"publisher":"National Academy of Sciences","issue":"21","license":[{"start":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T00:00:00Z","timestamp":1684108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM125749"],"award-info":[{"award-number":["R01GM125749"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM102779"],"award-info":[{"award-number":["GM102779"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,5,23]]},"abstract":"<jats:p>\n                    Synaptic transmission requires the coordinated activity of multiple synaptic proteins that are localized at the active zone (AZ).\u00a0We previously identified a\n                    <jats:italic>Caenorhabditis\u00a0elegans<\/jats:italic>\n                    protein named Clarinet (CLA-1) based on homology to the AZ proteins Piccolo, Rab3-interactingmolecule (RIM)\/UNC-10 and Fife. At the neuromuscular junction (NMJ),\n                    <jats:italic>cla-1 null<\/jats:italic>\n                    mutants exhibit release defects that are greatly exacerbated in\n                    <jats:italic>cla-1;unc-10 double<\/jats:italic>\n                    mutants. To gain insights into the coordinated roles of CLA-1 and UNC-10, we examined the relative contributions of each to the function and organization of the AZ.\u00a0Using a combination of electrophysiology, electron microscopy, and quantitative fluorescence imaging we explored the functional relationship of CLA-1 to other key AZ proteins including: RIM1, Cav2.1 channels, RIM1-binding protein, and Munc13 (\n                    <jats:italic>C. elegans<\/jats:italic>\n                    UNC-10, UNC-2, RIMB-1 and UNC-13, respectively). Our analyses show that CLA-1 acts in concert with UNC-10 to regulate UNC-2 calcium channel levels at the synapse via recruitment of\u00a0RIMB-1. In addition, CLA-1 exerts a RIMB-1-independent role in the localization of the priming factor UNC-13. Thus\n                    <jats:italic>C. elegans<\/jats:italic>\n                    CLA-1\/UNC-10 exhibit combinatorial effects that have overlapping design principles with other model organisms: RIM\/RBP and RIM\/ELKS in mouse and Fife\/RIM and BRP\/RBP in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    . These data support a semiconserved arrangement of AZ scaffolding proteins that are necessary for the localization and activation of the fusion machinery within nanodomains for precise coupling to Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2220856120","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T15:15:35Z","timestamp":1684163735000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":8,"title":["<i>C. elegans<\/i>\n                    Clarinet\/CLA-1 recruits RIMB-1\/RIM-binding protein and UNC-13 to orchestrate presynaptic neurotransmitter release"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6328-1477","authenticated-orcid":false,"given":"Mia","family":"Krout","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8203-5247","authenticated-orcid":false,"given":"Kelly H.","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy, Center for Cancer Cell Biology, Immunology, and Infection, Chicago Medical School, School of Graduate and Postdoctoral Studies, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ame","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy, Center for Cancer Cell Biology, Immunology, and Infection, Chicago Medical School, School of Graduate and Postdoctoral Studies, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8154-7774","authenticated-orcid":false,"given":"Elisa B.","family":"Frankel","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, New York, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peri T.","family":"Kurshan","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, New York, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4879-7122","authenticated-orcid":false,"given":"Hongkyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, New York, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet E.","family":"Richmond","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,5,15]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3137"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.23.090506.123417"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/14755"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057842"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn732"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201302022"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","unstructured":"Z. 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