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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,1,16]]},"abstract":"<jats:p>\n                    The prevalence of overweight and obesity continues to rise in the population worldwide. Because it is an important predisposing factor for cancer, cardiovascular diseases, diabetes mellitus, and COVID-19, obesity reduces life expectancy. Adipose tissue (AT), the main fat storage organ with endocrine capacity, plays fundamental roles in systemic metabolism and obesity-related diseases. Dysfunctional AT can induce excess or reduced body fat (lipodystrophy).\n                    <jats:italic>Dido1<\/jats:italic>\n                    is a marker gene for stemness; gene-targeting experiments compromised several functions ranging from cell division to embryonic stem cell differentiation, both in vivo and in vitro. We report that mutant mice lacking the DIDO N terminus show a lean phenotype. This consists of reduced AT and hypolipidemia, even when mice are fed a high-nutrient diet. DIDO mutation caused hypothermia due to lipoatrophy of white adipose tissue (WAT) and dermal fat thinning. Deep sequencing of the epididymal white fat (Epi WAT) transcriptome supported\n                    <jats:italic>Dido1<\/jats:italic>\n                    control of the cellular lipid metabolic process. We found that, by controlling the expression of transcription factors such as C\/EBP\u03b1 or PPAR\u03b3,\n                    <jats:italic>Dido1<\/jats:italic>\n                    is necessary for adipocyte differentiation, and that restoring their expression reestablished adipogenesis capacity in\n                    <jats:italic>Dido1<\/jats:italic>\n                    mutants. Our model differs from other lipodystrophic mice and could constitute a new system for the development of therapeutic intervention in obesity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2300096121","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T13:46:43Z","timestamp":1704808003000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":6,"title":["DIDO is necessary for the adipogenesis that promotes diet-induced obesity"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1417-9385","authenticated-orcid":false,"given":"Mar\u00eda \u00c1ngeles","family":"Garc\u00eda-L\u00f3pez","sequence":"first","affiliation":[{"name":"Department of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda-Consejo Superior de Investigaciones Cient\u00edficas Campus, Universidad Aut\u00f3noma de Madrid, Madrid 28049, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6397-4836","authenticated-orcid":false,"given":"Alfonso","family":"Mora","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaciones Cardiovasculares, Madrid 28029, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9817-8389","authenticated-orcid":false,"given":"Patricia","family":"Corrales","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon 28922, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7616-7750","authenticated-orcid":false,"given":"Tirso","family":"Pons","sequence":"additional","affiliation":[{"name":"Department of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda-Consejo Superior de Investigaciones Cient\u00edficas Campus, Universidad Aut\u00f3noma de Madrid, Madrid 28049, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6299-7588","authenticated-orcid":false,"given":"Ainhoa","family":"S\u00e1nchez de Diego","sequence":"additional","affiliation":[{"name":"Department of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda-Consejo Superior de Investigaciones Cient\u00edficas Campus, Universidad Aut\u00f3noma de Madrid, Madrid 28049, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0390-7428","authenticated-orcid":false,"given":"Amaia","family":"Talavera Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Department of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda-Consejo Superior de Investigaciones Cient\u00edficas Campus, Universidad Aut\u00f3noma de Madrid, Madrid 28049, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8431-8072","authenticated-orcid":false,"given":"Karel H. M.","family":"van Wely","sequence":"additional","affiliation":[{"name":"Department of Immunology and Oncology, Centro Nacional de Biotecnolog\u00eda-Consejo Superior de Investigaciones Cient\u00edficas Campus, Universidad Aut\u00f3noma de Madrid, Madrid 28049, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8169-681X","authenticated-orcid":false,"given":"Gema","family":"Medina-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences of Health, Area of Biochemistry and Molecular Biology, Universidad Rey Juan Carlos, Alcorcon 28922, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2822-0625","authenticated-orcid":false,"given":"Guadalupe","family":"Sabio","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaciones Cardiovasculares, Madrid 28029, 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