{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:50:41Z","timestamp":1791517841006,"version":"4.3.4"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"25","license":[{"start":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T00:00:00Z","timestamp":1686528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000997","name":"Arnold and Mabel Beckman Foundation","doi-asserted-by":"publisher","award":["Beckman Young investigator Award"],"award-info":[{"award-number":["Beckman Young investigator Award"]}],"id":[{"id":"10.13039\/100000997","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014185","name":"Searle Scholars Program","doi-asserted-by":"publisher","award":["Searle Scholars Progra"],"award-info":[{"award-number":["Searle Scholars Progra"]}],"id":[{"id":"10.13039\/100014185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015591","name":"Rose Hills Foundation","doi-asserted-by":"publisher","award":["Rose Hills Innovator Grant"],"award-info":[{"award-number":["Rose Hills Innovator Grant"]}],"id":[{"id":"10.13039\/100015591","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000008","name":"David and Lucile Packard Foundation","doi-asserted-by":"publisher","award":["Packard Fellowship in Science and Engineering"],"award-info":[{"award-number":["Packard Fellowship in Science and Engineering"]}],"id":[{"id":"10.13039\/100000008","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000893","name":"Simons Foundation","doi-asserted-by":"publisher","award":["Simons Early Career Investigator in Marine Microbial Ecology and Evolution"],"award-info":[{"award-number":["Simons Early Career Investigator in Marine Microbial Ecology and Evolution"]}],"id":[{"id":"10.13039\/100000893","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["Chan Zuckerberg Investigator"],"award-info":[{"award-number":["Chan Zuckerberg Investigator"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007247","name":"Adolph C. and Mary Sprague Miller Institute for Basic Research in Science, University of California Berkeley","doi-asserted-by":"publisher","award":["Miller Fellowship"],"award-info":[{"award-number":["Miller Fellowship"]}],"id":[{"id":"10.13039\/100007247","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["GM097142"],"award-info":[{"award-number":["GM097142"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,6,20]]},"abstract":"<jats:p>\n                    Methyl-coenzyme M reductase (MCR) catalyzes the formation of methane, and its activity accounts for nearly all biologically produced methane released into the atmosphere. The assembly of MCR is an intricate process involving the installation of a complex set of posttranslational modifications and the unique Ni-containing tetrapyrrole called coenzyme F\n                    <jats:sub>430<\/jats:sub>\n                    . Despite decades of research, details of MCR assembly remain largely unresolved. Here, we report the structural characterization of MCR in two intermediate states of assembly. These intermediate states lack one or both F\n                    <jats:sub>430<\/jats:sub>\n                    cofactors and form complexes with the previously uncharacterized McrD protein. McrD is found to bind asymmetrically to MCR, displacing large regions of the alpha subunit and increasing active-site accessibility for the installation of F\n                    <jats:sub>430<\/jats:sub>\n                    \u2014shedding light on the assembly of MCR and the role of McrD therein. This work offers crucial information for the expression of MCR in a heterologous host and provides targets for the design of MCR inhibitors.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2302815120","type":"journal-article","created":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T15:19:12Z","timestamp":1686583152000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":15,"title":["McrD binds asymmetrically to methyl-coenzyme M reductase improving active-site accessibility during assembly"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0700-9350","authenticated-orcid":false,"given":"Grayson L.","family":"Chadwick","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9049-2384","authenticated-orcid":false,"given":"Aaron M. N.","family":"Joiner","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9229-2009","authenticated-orcid":false,"given":"Sangeetha","family":"Ramesh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Illinois, Urbana, IL 61801"},{"name":"Carl R. Woese Institute for Genomic Biology, University of Illinois, Urbana, IL 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9564-0953","authenticated-orcid":false,"given":"Douglas A.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Illinois, Urbana, IL 61801"},{"name":"Carl R. Woese Institute for Genomic Biology, University of Illinois, Urbana, IL 61801"},{"name":"Department of Chemistry, University of Illinois, Urbana, IL 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipti D.","family":"Nayak","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,6,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2302815120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T15:22:37Z","timestamp":1686583357000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2302815120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,12]]},"references-count":0,"journal-issue":{"issue":"25","published-print":{"date-parts":[[2023,6,20]]}},"alternative-id":["10.1073\/pnas.2302815120"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2302815120","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.02.01.526716","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,12]]},"assertion":[{"value":"2023-02-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2302815120"}}