{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T23:31:40Z","timestamp":1793748700979,"version":"4.6.1"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"20","license":[{"start":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T00:00:00Z","timestamp":1683504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["17H01542"],"award-info":[{"award-number":["17H01542"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,5,16]]},"abstract":"<jats:p>\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    parasites, the causative agents of malaria, possess a complex lifecycle; however, the mechanisms of gene regulation involved in the cell-type changes remain unknown. Here, we report that gametocyte sucrose nonfermentable\u00a02 (gSNF2), an SNF2-like chromatin remodeling ATPase, plays an essential role in the differentiation of male gametocytes. Upon disruption of\n                    <jats:italic>gSNF2<\/jats:italic>\n                    , male gametocytes lost the capacity to develop into gametes. ChIP-seq analyses revealed that gSNF2 is widely recruited upstream of male-specific genes through a five-base, male-specific cis-acting element. In\n                    <jats:italic>gSNF2<\/jats:italic>\n                    -disrupted parasites, expression of over a hundred target genes was significantly decreased. ATAC-seq analysis demonstrated that decreased expression of these genes correlated with a decrease of the nucleosome-free region upstream of these genes. These results suggest that global changes induced in the chromatin landscape by gSNF2 are the initial step in male differentiation from early gametocytes. This study provides the possibility that chromatin remodeling is responsible for cell-type changes in the\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    lifecycle.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2303432120","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T15:17:50Z","timestamp":1683559070000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":12,"title":["Differentiation of\n                    <i>Plasmodium<\/i>\n                    male gametocytes is initiated by the recruitment of a chromatin remodeler to a male-specific cis-element"],"prefix":"10.1073","volume":"120","author":[{"given":"Izumi","family":"Kaneko","sequence":"first","affiliation":[{"name":"Department of Medical Zoology, Mie University School of Medicine, Mie, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsubasa","family":"Nishi","sequence":"additional","affiliation":[{"name":"Department of Medical Zoology, Mie University School of Medicine, Mie, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiroh","family":"Iwanaga","sequence":"additional","affiliation":[{"name":"Department of Molecular Protozoology, Research Center for Infectious Disease Control, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3416-5132","authenticated-orcid":false,"given":"Masao","family":"Yuda","sequence":"additional","affiliation":[{"name":"Department of Medical Zoology, Mie University School of Medicine, Mie, Tsu 514-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,5,8]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","unstructured":"C. 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Yuda Differentiation of malaria male gametocytes is initiated by recruitment of a chromatin remodeler to male-specific cis-acting elements. Gene Expression Omnibus. https:\/\/www.ncbi.xyz\/geo\/query\/acc.cgi?acc=GSE198588. 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