{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:45:31Z","timestamp":1791917131897,"version":"4.3.4"},"reference-count":56,"publisher":"National Academy of Sciences","issue":"29","license":[{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["BFU2017-83720-P PID2020-120141GB-I00 and MDM-2014-0435"],"award-info":[{"award-number":["BFU2017-83720-P PID2020-120141GB-I00 and MDM-2014-0435"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Detroit Mercy School of Dentistry","award":["FRG-2017-02"],"award-info":[{"award-number":["FRG-2017-02"]}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["RL5GM118981 TL4GM118983 and 1UL1GM118982"],"award-info":[{"award-number":["RL5GM118981 TL4GM118983 and 1UL1GM118982"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,7,18]]},"abstract":"<jats:p>\n                    ToxR, a\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    transmembrane one-component signal transduction factor, lies within a regulatory cascade that results in the expression of ToxT, toxin coregulated pilus, and cholera toxin. While ToxR has been extensively studied for its ability to activate or repress various genes in\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    , here we present the crystal structures of the ToxR cytoplasmic domain bound to DNA at the\n                    <jats:italic>toxT<\/jats:italic>\n                    and\n                    <jats:italic>ompU<\/jats:italic>\n                    promoters. The structures confirm some predicted interactions, yet reveal other unexpected promoter interactions with implications for other potential regulatory roles for ToxR. We show that ToxR is a versatile virulence regulator that recognizes diverse and extensive, eukaryotic-like regulatory DNA sequences, that relies more on DNA structural elements than specific sequences for binding. Using this topological DNA recognition mechanism, ToxR can bind both in tandem and in a twofold inverted-repeat-driven manner. Its regulatory action is based on coordinated multiple binding to promoter regions near the transcription start site, which can remove the repressing H-NS proteins and prepares the DNA for optimal interaction with the RNA polymerase.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2304378120","type":"journal-article","created":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T15:06:39Z","timestamp":1689001599000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":19,"title":["ToxR activates the\n                    <i>Vibrio cholerae<\/i>\n                    virulence genes by tethering DNA to the membrane through versatile binding to multiple sites"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0429-9348","authenticated-orcid":false,"given":"Albert","family":"Canals","sequence":"first","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain"},{"name":"Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Cient\u00edficas, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Pieretti","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain"},{"name":"Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Cient\u00edficas, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireia","family":"Muriel-Masanes","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain"},{"name":"Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Cient\u00edficas, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nour","family":"El Yaman","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Detroit Mercy, Detroit, MI 48221"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah C.","family":"Plecha","sequence":"additional","affiliation":[{"name":"Division of Integrated Biomedical Sciences, University of Detroit Mercy School of Dentistry, Detroit, MI 48208"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3267-2632","authenticated-orcid":false,"given":"Joshua J.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Division of Integrated Biomedical Sciences, University of Detroit Mercy School of Dentistry, Detroit, MI 48208"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"F\u00e0brega-Ferrer","sequence":"additional","affiliation":[{"name":"Institute for Research in Biomedicine (IRB 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