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The resulting orange-emitting HVI\u2013COT\u2013Cy3 enables high-fidelity voltage imaging for up to 30 min in cultured primary neurons with a sensitivity of ~ \u221230% \u0394F\/F\n                    <jats:sub>0<\/jats:sub>\n                    per action potential (AP). It also maximally preserves the signal of individual APs in cardiomyocytes. The far-red-emitting HVI\u2013COT\u2013Cy5 allows two-color voltage\/calcium imaging with GCaMP6s in neurons and cardiomyocytes for 15 min. We leverage the HVI\u2013COT series with reduced phototoxicity and photobleaching to evaluate the impact of drug candidates on the electrophysiology of excitable cells.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2306950120","type":"journal-article","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T14:06:48Z","timestamp":1692281208000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":20,"title":["Orange\/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes"],"prefix":"10.1073","volume":"120","author":[{"given":"Shuzhang","family":"Liu","sequence":"first","affiliation":[{"name":"College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, Peking University, Beijing 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