{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T06:37:34Z","timestamp":1784615854931,"version":"3.55.0"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"44","license":[{"start":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:00:00Z","timestamp":1698019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"HHS | NIH | National Cancer Institute","doi-asserted-by":"publisher","award":["R35-CA197706"],"award-info":[{"award-number":["R35-CA197706"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,10,31]]},"abstract":"<jats:p>Chronic lymphocytic leukemia (CLL) is one of the most diagnosed forms of leukemia worldwide and it is usually classified into two forms: indolent and aggressive. These two forms are characterized by distinct molecular features that drive different responses to treatment and clinical outcomes. In this context, a better understanding of the molecular landscape of the CLL forms may potentially lead to the development of new drugs or the identification of novel biomarkers. Human endogenous retroviruses (HERVs) are a class of transposable elements that have been associated with the development of different human cancers, including different forms of leukemias. However, no studies about HERVs in CLL have ever been reported so far. Here, we present the first locus-specific profiling of HERV expression in both the aggressive and indolent forms of CLL. Our analyses revealed several dysregulations in HERV expression occurring in CLL and some of them were specific for either the aggressive or indolent form of CLL. Such results were also validated by analyzing an external cohort of CLL patients and by RT-qPCR. Moreover, in silico analyses have shown relevant signaling pathways associated with them suggesting a potential involvement of the dysregulated HERVs in these pathways and consequently in CLL development.<\/jats:p>","DOI":"10.1073\/pnas.2307593120","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T15:03:42Z","timestamp":1698073422000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":14,"title":["Expression signature of human endogenous retroviruses in chronic lymphocytic leukemia"],"prefix":"10.1073","volume":"120","author":[{"given":"Alessandro La","family":"Ferlita","sequence":"first","affiliation":[{"name":"Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Nigita","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liudmyla","family":"Tsyba","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey","family":"Palamarchuk","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Alaimo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Bioinformatics Unit, University of Catania, Catania 95123, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Pulvirenti","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Bioinformatics Unit, University of Catania, Catania 95123, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Balatti","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rassenti","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Diego, La Jolla CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip N.","family":"Tsichlis","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kipps","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Diego, La Jolla CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Pekarsky","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3788-1457","authenticated-orcid":false,"given":"Carlo M.","family":"Croce","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology and Genetics, The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,10,23]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2010.09.003"},{"key":"e_1_3_4_2_2","first-page":"9","volume":"2","author":"Bullrich F.","year":"2001","unstructured":"F. Bullrich, C. M. Croce, Molecular biology of chronic lymphocytic leukemia. Basic and Clinical Oncology 2, 9\u201332 (2001).","journal-title":"Basic and Clinical Oncology"},{"key":"e_1_3_4_3_2","first-page":"1","article-title":"Chronic Lymphocytic Leukemia: Epidemiological, Familial, and Genetic Aspects","volume":"2","author":"Cheson B. D.","year":"2001","unstructured":"B. D. Cheson, Chronic Lymphocytic Leukemia: Epidemiological, Familial, and Genetic Aspects. CLL 2, 1\u201330 (2001).","journal-title":"CLL"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.96"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2018.12.002"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-6838"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.02.013"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13027-019-0260-7"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aat9820"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12070726"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41525-021-00177-w"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2021-004241"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.820883"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11010095"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-21-0806"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-21-3523"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13258-022-01280-7"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202152984"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05771-9"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14184433"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02039"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.42.110807.091501"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1602336113"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001826"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1814203116"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01258-17"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7030939"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307885100"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1401215111"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17206-4"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.637981"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2022-162265"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1006453"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09645-5"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9788"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02357-4"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1604266113"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_3_4_41_2","unstructured":"K. Blighe S. Rana M. Lewis EnhancedVolcano: Publication-ready volcano plots with enhanced colouring and labeling. R package version (2018). https:\/\/github.com\/kevinblighe\/EnhancedVolcano."},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-015-0611-3"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu393"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0201-4"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2307593120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T15:07:19Z","timestamp":1698073639000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2307593120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,23]]},"references-count":47,"journal-issue":{"issue":"44","published-print":{"date-parts":[[2023,10,31]]}},"alternative-id":["10.1073\/pnas.2307593120"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2307593120","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,23]]},"assertion":[{"value":"2023-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2307593120"}}