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U.S.A."],"published-print":{"date-parts":[[2023,9,26]]},"abstract":"<jats:p>\n                    <jats:italic>Vibrio cholerae,<\/jats:italic>\n                    the causative agent of the disease cholera, is responsible for multiple pandemics.\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    binds to and colonizes the gastrointestinal tract within the human host, as well as various surfaces in the marine environment (e.g., zooplankton) during interepidemic periods. A large adhesin, the Flagellar Regulated Hemagglutinin A (FrhA), enhances binding to erythrocytes and epithelial cells and enhances intestinal colonization. We identified a peptide-binding domain (PBD) within FrhA that mediates hemagglutination, binding to epithelial cells, intestinal colonization, and facilitates biofilm formation. Intriguingly, this domain is also found in the ice-binding protein of the Antarctic bacterium\n                    <jats:italic>Marinomonas primoryensis<\/jats:italic>\n                    , where it mediates binding to diatoms. Peptide inhibitors of the\n                    <jats:italic>M. primoryensis<\/jats:italic>\n                    PBD inhibit\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    binding to human cells as well as to diatoms and inhibit biofilm formation. Moreover, the\n                    <jats:italic>M. primoryensis<\/jats:italic>\n                    PBD inserted into FrhA allows\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    to bind human cells and colonize the intestine and also enhances biofilm formation, demonstrating the interchangeability of the PBD from these bacteria. Importantly, peptide inhibitors of PBD reduce\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    intestinal colonization in infant mice. These studies demonstrate how\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    uses a PBD shared with a diatom-binding Antarctic bacterium to facilitate intestinal colonization in humans and biofilm formation in the environment.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2308238120","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T13:31:57Z","timestamp":1695216717000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":15,"title":["A peptide-binding domain shared with an Antarctic bacterium facilitates\n                    <i>Vibrio cholerae<\/i>\n                    human cell binding and intestinal colonization"],"prefix":"10.1073","volume":"120","author":[{"given":"Cameron J.","family":"Lloyd","sequence":"first","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX 78249"},{"name":"Department of Molecular Microbiology and Immunology, University of Texas, San Antonio, TX 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaiqi","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, Queen\u2019s University, Kingston, ON K7L 3N6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Kinrade","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, Queen\u2019s University, Kingston, ON K7L 3N6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Zahiri","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, Queen\u2019s University, Kingston, ON K7L 3N6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0377-1073","authenticated-orcid":false,"given":"Robert","family":"Eves","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, Queen\u2019s University, Kingston, ON K7L 3N6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3613-0248","authenticated-orcid":false,"given":"Syed Khalid","family":"Ali","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX 78249"},{"name":"Department of Molecular Microbiology and Immunology, University of Texas, San Antonio, TX 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6384-7167","authenticated-orcid":false,"given":"Fitnat","family":"Yildiz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3543-4821","authenticated-orcid":false,"given":"Ilja K.","family":"Voets","sequence":"additional","affiliation":[{"name":"Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology, Eindhoven 5612, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter L.","family":"Davies","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, Queen\u2019s University, Kingston, ON K7L 3N6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2007-1407","authenticated-orcid":false,"given":"Karl E.","family":"Klose","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX 78249"},{"name":"Department of Molecular Microbiology and Immunology, University of Texas, San Antonio, TX 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,9,20]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"97","article-title":"Cholera toxin: Genetic analysis, regulation, and role in pathogenesis","volume":"118","author":"Mekalanos J. 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