{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T01:12:02Z","timestamp":1788484322485,"version":"build-2803163510"},"reference-count":84,"publisher":"National Academy of Sciences","issue":"36","license":[{"start":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T00:00:00Z","timestamp":1693180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/04684-4"],"award-info":[{"award-number":["2014\/04684-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/11342-6"],"award-info":[{"award-number":["2019\/11342-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/07870-9"],"award-info":[{"award-number":["2016\/07870-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"DHAC | National Health and Medical Research Council","doi-asserted-by":"publisher","award":["2010841"],"award-info":[{"award-number":["2010841"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,9,5]]},"abstract":"<jats:p>\n                    The causative agent of human Q fever,\n                    <jats:italic>Coxiella burnetii,<\/jats:italic>\n                    is highly adapted to infect alveolar macrophages by inhibiting a range of host responses to infection. Despite the clinical and biological importance of this pathogen, the challenges related to genetic manipulation of both\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    and macrophages have limited our knowledge of the mechanisms by which\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    subverts macrophages functions. Here, we used the related bacterium\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    to perform a comprehensive screen of\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    effectors that interfere with innate immune responses and host death using the greater wax moth\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    and mouse bone marrow\u2013derived macrophages. We identified MceF (Mitochondrial Coxiella effector protein F), a\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    effector protein that localizes to mitochondria and contributes to host cell survival. MceF was shown to enhance mitochondrial function, delay membrane damage, and decrease mitochondrial ROS production induced by rotenone. Mechanistically, MceF recruits the host antioxidant protein Glutathione Peroxidase 4 (GPX4) to the mitochondria. The protective functions of MceF were absent in primary macrophages lacking GPX4, while overexpression of MceF in human cells protected against oxidative stress\u2013induced cell death.\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    lacking MceF was replication competent in mammalian cells but induced higher mortality in\n                    <jats:italic>G. mellonella,<\/jats:italic>\n                    indicating that MceF modulates the host response to infection. This study reveals an important\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    strategy to subvert macrophage cell death and host immunity and demonstrates that modulation of the host antioxidant system is a viable strategy to promote the success of intracellular bacteria.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2308752120","type":"journal-article","created":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T15:12:27Z","timestamp":1693235547000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":14,"title":["<i>Coxiella<\/i>\n                    co-opts the Glutathione Peroxidase 4 to protect the host cell from oxidative stress\u2013induced cell death"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8645-5158","authenticated-orcid":false,"given":"Robson K.","family":"Loterio","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto-SP 14049-900, Brazil"},{"name":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia"},{"name":"Infection Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warrison","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto-SP 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi Wei","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo L.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto-SP 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle P. A.","family":"Mascarenhas","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto-SP 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago M.","family":"Steiner","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica","family":"Chiaratto","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto-SP 14040-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura F.","family":"Fielden","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia"},{"name":"Department of Biochemistry and Pharmacology and the Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3115-1066","authenticated-orcid":false,"given":"Leticia","family":"Lopes","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto-SP 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7082-3115","authenticated-orcid":false,"given":"Lauren E.","family":"Bird","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo H.","family":"Goldman","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto-SP 14040-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0199-3222","authenticated-orcid":false,"given":"Diana","family":"Stojanovski","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Pharmacology and the Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2556-8316","authenticated-orcid":false,"given":"Nichollas E.","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7856-7512","authenticated-orcid":false,"given":"Dario S.","family":"Zamboni","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto-SP 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9240-2001","authenticated-orcid":false,"given":"Hayley J.","family":"Newton","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC 3000, Australia"},{"name":"Infection Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,8,28]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftab052"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.3MR0916-380R"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00411-16"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10205"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12432"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/23744235.2018.1445279"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2017.09.010"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00175-11"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002056"},{"key":"e_1_3_4_10_2","first-page":"243","article-title":"Beginning to understand the role of the Type IV secretion system effector proteins in Coxiella burnetii pathogenesis","volume":"413","author":"L\u00fchrmann A.","year":"2017","unstructured":"A. L\u00fchrmann, H. J. Newton, M. Bonazzi, Beginning to understand the role of the Type IV secretion system effector proteins in Coxiella burnetii pathogenesis. Curr. Top Microbiol. Immunol. 413, 243\u2013268 (2017).","journal-title":"Curr. Top Microbiol. Immunol."},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03626.x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/15687"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501223"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00071-13"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01729-14"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1215278110"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010485107"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00043"},{"key":"e_1_3_4_19_2","first-page":"619","article-title":"Defying death\u2013How Coxiella burnetii copes with intentional host cell suicide","volume":"92","author":"Cordsmeier A.","year":"2019","unstructured":"A. Cordsmeier, N. Wagner, A. L\u00fchrmann, C. Berens, Defying death\u2013How Coxiella burnetii copes with intentional host cell suicide. Yale J. Biol. Med. 92, 619\u2013628 (2019).","journal-title":"Yale J. Biol. Med."},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01124-08"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00863-07"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004380107"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12066"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","unstructured":"A. Cordsmeier The Coxiella burnetii T4SS effector protein AnkG hijacks the 7SK small nuclear ribonucleoprotein complex for reprogramming host cell transcription. PLoS Pathog. 18 e1010266 (2022). e1010266","DOI":"10.1371\/journal.ppat.1010266"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201899753"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.077230-0"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","unstructured":"E. Martinez Coxiella burnetii effector CvpB modulates phosphoinositide metabolism for optimal vacuole development. Proc. Natl. Acad. Sci. U.S.A. 113 E3260\u2013E3269 (2016) 10.1073\/pnas.1522811113.","DOI":"10.1073\/pnas.1522811113"},{"key":"e_1_3_4_28_2","first-page":"1","article-title":"Effector protein cig2 decreases host tolerance of infection by directing constitutive fusion of autophagosomes with the Coxiella-containing vacuole","volume":"7","author":"Kohler L. J.","year":"2016","unstructured":"L. J. Kohler , Effector protein cig2 decreases host tolerance of infection by directing constitutive fusion of autophagosomes with the Coxiella-containing vacuole. mBio 7, 1\u201314 (2016).","journal-title":"mBio"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00913-19"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402096200"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01046-16"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003784"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA120.002370"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.17463"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep45465"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1700258"},{"key":"e_1_3_4_37_2","first-page":"3","article-title":"Modified annexin V\/propidium iodide apoptosis assay for accurate assessment of cell death","volume":"50","author":"Rieger A. M.","year":"2011","unstructured":"A. M. Rieger, K. L. Nelson, J. D. Konowalchuk, D. R. Barreda, Modified annexin V\/propidium iodide apoptosis assay for accurate assessment of cell death. J. Visualized Exp. 50, 3\u20136 (2011).","journal-title":"J. Visualized Exp."},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0199938"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-019-0164-5"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.09.033"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.04.024"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1261-2_19"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2015.00083"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/217670"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304854200"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21280"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.05.012"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.05.029"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.03.045"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.10.086"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3510183"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7115"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.41.29294"},{"key":"e_1_3_4_55_2","unstructured":"D. Tang Ferroptosis in Health and Disease (Springer Nature Switzerland 2019) 10.1007\/978-3-030-26780-3."},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M410387200"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.05.009"},{"key":"e_1_3_4_58_2","first-page":"154","article-title":"GPx4 in bacterial infection and polymicrobial sepsis: Involvement of ferroptosis and pyroptosis","volume":"7","author":"Zhu H.","year":"2019","unstructured":"H. Zhu, A. Santo, Z. Jia, Y. Li, GPx4 in bacterial infection and polymicrobial sepsis: Involvement of ferroptosis and pyroptosis. React. Oxyg. Species 7, 154\u2013160 (2019).","journal-title":"React. Oxyg. Species"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09663"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA119.001808"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.06.028"},{"key":"e_1_3_4_62_2","first-page":"1225","article-title":"Nitric oxide partially controls","volume":"71","author":"Zamboni D. S.","year":"2003","unstructured":"D. S. Zamboni, M. Rabinovitch, Nitric oxide partially controls. Am. Soc. Mcrobiol. 71, 1225\u20131233 (2003).","journal-title":"Am. Soc. Mcrobiol."},{"key":"e_1_3_4_63_2","first-page":"1","article-title":"Critical role for molecular iron in Coxiella burnetii replication and viability","volume":"5","author":"Sanchez S. E.","year":"2020","unstructured":"S. E. Sanchez, A. Omsland, Critical role for molecular iron in Coxiella burnetii replication and viability. Am. J. Public Health 5, 1\u201317 (2020).","journal-title":"Am. J. Public Health"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.005190"},{"key":"e_1_3_4_65_2","first-page":"1","article-title":"SdrA, an NADP(H)-regenerating enzyme, is crucial for Coxiella burnetii to resist oxidative stress and replicate intracellularly","volume":"22","author":"Bitew M. A.","year":"2019","unstructured":"M. A. Bitew , SdrA, an NADP(H)-regenerating enzyme, is crucial for Coxiella burnetii to resist oxidative stress and replicate intracellularly. Cell Microbiol. 22, 1\u201316 (2019).","journal-title":"Cell Microbiol."},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.154.1.520-523.1983"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0430-8"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.04.005"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00608-17"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101107"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1603\/029.102.0122"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2009.04.008"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002083"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015263"},{"key":"e_1_3_4_75_2","doi-asserted-by":"crossref","unstructured":"A. V. Gon\u00e7alves Gasdermin-D and caspase-7 are the key caspase-1\/8 substrates downstream of the NAIP5\/NLRC4 inflammasome required for restriction of Legionella pneumophila . PLoS Pathog. 15 e1007886 (2019). e1007886","DOI":"10.1371\/journal.ppat.1007886"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006502"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004286"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00881-12"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3221"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205613200"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1145"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1106"},{"key":"e_1_3_4_84_2","unstructured":"R. Loterio N. Scott H. Newton CBU1543 immunoprecipation (CBU1543 vs empty vector). PRICE Proteomics Identifications Database. http:\/\/www.ebi.ac.uk\/pride\/archive\/projects\/PXD030191. Deposited 5 December 2021."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2308752120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T09:28:32Z","timestamp":1695288512000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2308752120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,28]]},"references-count":84,"journal-issue":{"issue":"36","published-print":{"date-parts":[[2023,9,5]]}},"alternative-id":["10.1073\/pnas.2308752120"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2308752120","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,28]]},"assertion":[{"value":"2023-06-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2308752120"}}