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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,7,9]]},"abstract":"<jats:p>Tissue buckling is an increasingly appreciated mode of morphogenesis in the embryo, but it is often unclear how geometric and material parameters are molecularly determined in native developmental contexts to generate diverse functional patterns. Here, we study the link between differential mechanical properties and the morphogenesis of distinct anteroposterior compartments in the intestinal tract\u2014the esophagus, small intestine, and large intestine. These regions originate from a simple, common tube but adopt unique forms. Using measured data from the developing chick gut coupled with a minimal theory and simulations of differential growth, we investigate divergent lumen morphologies along the entire early gut and demonstrate that spatiotemporal geometries, moduli, and growth rates control the segment-specific patterns of mucosal buckling. Primary buckling into wrinkles, folds, and creases along the gut, as well as secondary buckling phenomena, including period-doubling in the foregut and multiscale creasing-wrinkling in the hindgut, are captured and well explained by mechanical models. This study advances our existing knowledge of how identity leads to form in these regions, laying the foundation for future work uncovering the relationship between molecules and mechanics in gut morphological regionalization.<\/jats:p>","DOI":"10.1073\/pnas.2310992121","type":"journal-article","created":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T12:55:15Z","timestamp":1720184115000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":29,"title":["The developmental mechanics of divergent buckling patterns in the chick gut"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0718-5920","authenticated-orcid":false,"given":"Hasreet K.","family":"Gill","sequence":"first","affiliation":[{"name":"Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0296-3981","authenticated-orcid":false,"given":"Sifan","family":"Yin","sequence":"additional","affiliation":[{"name":"Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Lawlor","sequence":"additional","affiliation":[{"name":"Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler R.","family":"Huycke","sequence":"additional","affiliation":[{"name":"Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115"},{"name":"Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1309-8919","authenticated-orcid":false,"given":"Nandan L.","family":"Nerurkar","sequence":"additional","affiliation":[{"name":"The Fu Foundation School of Engineering and Applied Science, Department of Biomedical Engineering, Columbia University, New York, NY 10027"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clifford J.","family":"Tabin","sequence":"additional","affiliation":[{"name":"Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5114-0519","authenticated-orcid":false,"given":"L.","family":"Mahadevan","sequence":"additional","affiliation":[{"name":"Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138"},{"name":"Department of Physics, Harvard University, Cambridge, MA 02138"},{"name":"Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"e_1_3_5_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-019-03102-x"},{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22540"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.10.3163"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2011.07.005"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3608"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1070310305"},{"key":"e_1_3_5_7_2","volume-title":"Biology of the Reptilia","author":"Wake D. 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