{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:49:02Z","timestamp":1792604942325,"version":"4.5.0"},"reference-count":66,"publisher":"National Academy of Sciences","issue":"2","license":[{"start":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T00:00:00Z","timestamp":1704240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI143916"],"award-info":[{"award-number":["R01AI143916"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI077628"],"award-info":[{"award-number":["R01AI077628"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI134895"],"award-info":[{"award-number":["R01AI134895"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,1,9]]},"abstract":"<jats:p>\n                    Bacterial infections are often polymicrobial.\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    cause chronic co-infections, which are more problematic than mono-species infections. Understanding the mechanisms of their interactions is crucial for treating co-infections. Staphyloxanthin (STX), a yellow pigment synthesized by the\n                    <jats:italic>S. aureus crt<\/jats:italic>\n                    operon, promotes\n                    <jats:italic>S. aureus<\/jats:italic>\n                    resistance to oxidative stress and neutrophil-mediated killing. We found that STX production by\n                    <jats:italic>S. aureus<\/jats:italic>\n                    , either as surface-grown macrocolonies or planktonic cultures, was elevated when exposed to the\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    exoproduct, 2-heptyl-4-hydroxyquinoline N-oxide (HQNO). This was observed with both mucoid and non-mucoid\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    strains. The induction phenotype was found in a majority of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>S. aureus<\/jats:italic>\n                    clinical isolates examined. When subjected to hydrogen peroxide or human neutrophils,\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    survival was significantly higher when mixed with wild-type (WT)\n                    <jats:italic>S. aureus<\/jats:italic>\n                    , compared to\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    alone or with an\n                    <jats:italic>S. aureus crt<\/jats:italic>\n                    mutant deficient in STX production. In a murine wound model, co-infection with WT\n                    <jats:italic>S. aureus<\/jats:italic>\n                    , but not the STX-deficient mutant, enhanced\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    burden and disease compared to mono-infection. In conclusion, we identified a role for\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    HQNO mediating polymicrobial interactions with\n                    <jats:italic>S. aureus<\/jats:italic>\n                    by inducing STX production, which consequently promotes resistance to the innate immune effectors H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and neutrophils. These results further our understanding of how different bacterial species cooperatively cause co-infections.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2312334121","type":"journal-article","created":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T13:44:21Z","timestamp":1704289461000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":20,"title":["A bacterial pigment provides cross-species protection from H\n                    <sub>2<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    - and neutrophil-mediated killing"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4136-8146","authenticated-orcid":false,"given":"Yiwei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Microbiology, Ohio State University, Columbus, OH 43210"},{"name":"Department of Microbial Infection and Immunity, Ohio State University College of Medicine, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanor A.","family":"McQuillen","sequence":"additional","affiliation":[{"name":"Department of Health and Rehabilitation Sciences, Ohio State University College of Medicine, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranav S. J. B.","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ohio State University, Columbus, OH 43210"},{"name":"Department of Microbial Infection and Immunity, Ohio State University College of Medicine, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8895-3444","authenticated-orcid":false,"given":"Erin S.","family":"Gloag","sequence":"additional","affiliation":[{"name":"Department of Microbial Infection and Immunity, Ohio State University College of Medicine, Columbus, OH 43210"},{"name":"Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2932-7966","authenticated-orcid":false,"given":"Matthew R.","family":"Parsek","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4592-4816","authenticated-orcid":false,"given":"Daniel J.","family":"Wozniak","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ohio State University, Columbus, OH 43210"},{"name":"Department of Microbial Infection and Immunity, Ohio State University College of Medicine, Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,1,3]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201306-159MG"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11951"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056846"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2015.1023291"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2621-0"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2012.05.028"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53203-8"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.2.554-566.2005"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2012.10.064"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00076-22"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2011.01121.x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0630130"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606756104"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307694100"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/fty003"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00538-16"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.83"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-017-0035-0"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.147.3.900-913.1981"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.24.7719-7726.1994"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.06.006"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00598"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050846"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00680-10"},{"key":"e_1_3_4_25_2","first-page":"175","article-title":"Pseudomonas aeruginosa induces pigment production and enhances virulence in a white phenotypic variant of Staphylococcus aureus","volume":"6","author":"Antonic V.","year":"2013","unstructured":"V. Antonic, A. Stojadinovic, B. Zhang, M. J. Izadjoo, M. Alavi, Pseudomonas aeruginosa induces pigment production and enhances virulence in a white phenotypic variant of Staphylococcus aureus. Infect. Drug Resist. 6, 175\u2013186 (2013).","journal-title":"Infect. Drug Resist."},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000983"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01140-07"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2004.02.012"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA117.000789"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-33"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00047-21"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00186-17"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00358-21"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129670"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086705"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.845231"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.60.3.539-574.1996"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00204-06"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-012-0229-x"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00373"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00286"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00234-19"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/30.5.615"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00059-15"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00099"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01138-07"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00559-19"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00426-20"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00116-17"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2212340119"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(77)90014-7"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00067a033"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.11.056"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02331-8"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00033"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-7-99"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.910-919.2006"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027317"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-30"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1999.1441"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00537-12"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1419677112"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-467-4_2"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01704.x"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66823-y"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.4360"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2312334121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T13:44:45Z","timestamp":1704289485000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2312334121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,3]]},"references-count":66,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,1,9]]}},"alternative-id":["10.1073\/pnas.2312334121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2312334121","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,3]]},"assertion":[{"value":"2023-07-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2312334121"}}