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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,7,2]]},"abstract":"<jats:p>\n                    Controlling the principal African malaria vector, the mosquito\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    , is considered essential to curtail malaria transmission. However, existing vector control technologies rely on insecticides, which are becoming increasingly ineffective. Sterile insect technique (SIT) is a powerful suppression approach that has successfully eradicated a number of insect pests, yet the\n                    <jats:italic>A. gambiae<\/jats:italic>\n                    toolkit lacks the requisite technologies for its implementation. SIT relies on iterative mass releases of nonbiting, nondriving, sterile males which seek out and mate with monandrous wild females. Once mated, females are permanently sterilized due to mating-induced refractoriness, which results in population suppression of the subsequent generation. However, sterilization by traditional methods renders males unfit, making the creation of precise genetic sterilization methods imperative. Here, we introduce a vector control technology termed precision-guided sterile insect technique (pgSIT), in\n                    <jats:italic>A. gambiae<\/jats:italic>\n                    for inducible, programmed male sterilization and female elimination for wide-scale use in SIT campaigns. Using a binary CRISPR strategy, we cross separate engineered Cas9 and gRNA strains to disrupt male-fertility and female-essential genes, yielding &gt;99.5% male sterility and &gt;99.9% female lethality in hybrid progeny. We demonstrate that these genetically sterilized males have good longevity, are able to induce sustained population suppression in cage trials, and are predicted to eliminate wild\n                    <jats:italic>A. gambiae<\/jats:italic>\n                    populations using mathematical models, making them ideal candidates for release. This work provides a valuable addition to the malaria genetic biocontrol toolkit, enabling scalable SIT-like confinable, species-specific, and safe suppression in the species.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2312456121","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T12:43:44Z","timestamp":1719319424000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":43,"title":["Eliminating malaria vectors with precision-guided sterile males"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2059-4614","authenticated-orcid":false,"given":"Reema A.","family":"Apte","sequence":"first","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea L.","family":"Smidler","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7247-5142","authenticated-orcid":false,"given":"James J.","family":"Pai","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha L.","family":"Chow","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanle","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3369-987X","authenticated-orcid":false,"given":"Agastya","family":"Mondal","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, School of Public Health, University of California, Berkeley, CA 94720"},{"name":"Division of Biostatistics, School of Public Health, University of California, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor M.","family":"S\u00e1nchez C.","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, School of Public Health, University of California, Berkeley, CA 94720"},{"name":"Division of Biostatistics, School of Public Health, University of California, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Antoshechkin","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0603-7341","authenticated-orcid":false,"given":"John M.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, School of Public Health, University of California, Berkeley, CA 94720"},{"name":"Division of Biostatistics, School of Public Health, University of California, Berkeley, CA 94720"},{"name":"Innovative Genomics Institute, University of California, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6853-9884","authenticated-orcid":false,"given":"Omar S.","family":"Akbari","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"e_1_3_4_1_2","unstructured":"World Health Organization \u201cWorld Malaria Report 2021\u201d (WHO 2021)."},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-022-04343-7"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","unstructured":"O. 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Zha \u201cPredictive models for visually classifying insects.\u201d US Patent 17863802.9 (2021)."},{"key":"e_1_3_4_50_2","unstructured":"H. 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