{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:32:14Z","timestamp":1787236334641,"version":"build-2736575974"},"reference-count":15,"publisher":"National Academy of Sciences","issue":"47","license":[{"start":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:00:00Z","timestamp":1699833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006955","name":"HHS | NIH | Office of Extramural Research, National Institutes of Health","doi-asserted-by":"publisher","award":["R35 GM142898"],"award-info":[{"award-number":["R35 GM142898"]}],"id":[{"id":"10.13039\/100006955","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,11,21]]},"abstract":"<jats:p>\n                    A model for antibiotic accumulation in bacterial biofilm microcolonies utilizing heterogenous porosity and attachment site profiles replicated the periphery sequestration reported in prior experimental studies on\n                    <jats:italic>Pseudomonas aeruginosa PAO1<\/jats:italic>\n                    biofilm cell clusters. These\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    cell clusters are in vitro models of the chronic\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    infections in cystic fibrosis patients which display recalcitrance to antibiotic treatments, leading to exacerbated morbidity and mortality. This resistance has been partially attributed to periphery sequestration, where antibiotics fail to penetrate biofilm cell clusters. The physical phenomena driving this periphery sequestration have not been definitively established. This paper introduces mathematical models to account for two proposed physical phenomena driving periphery sequestration: biofilm matrix attachment and volume-exclusion due to variable biofilm porosity. An antibiotic accumulation model which incorporated these phenomena better fit observed periphery sequestration data compared to previous models.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2312995120","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T15:08:51Z","timestamp":1699888131000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":8,"title":["Heterogenous biofilm mass-transport model replicates periphery sequestration of antibiotics in\n                    <i>Pseudomonas aeruginosa<\/i>\n                    PAO1 microcolonies"],"prefix":"10.1073","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6050-9544","authenticated-orcid":false,"given":"Joshua","family":"Prince","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, Duke University, Durham, NC 27708"},{"name":"Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3840-8039","authenticated-orcid":false,"suffix":"III","given":"A-Andrew D.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Duke University, Durham, NC 27708"},{"name":"Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,11,13]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0042-1758732"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00682-4"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00307-19"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6NR09736G"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.221120"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.102.032408"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00001-13"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12155"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.11.2517"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1061\/(ASCE)0733-9372(1996)122:11(1023)"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/npjbiofilms.2016.12"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0261482"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.4890740"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24390"},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","unstructured":"J. Prince A. -A. D. Jones III Data and scripts from: Heterogenous biofilm mass-transport model replicates perphery sequestration of antibiotics in Pseudomonas aeruginosa PAO1 microcolonies. Duke Research Data Repository. https:\/\/dx.doi.org\/10.7924\/r4pr80j7f. Deposited 30 October 2023.","DOI":"10.1101\/2023.07.28.551018"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2312995120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T15:10:44Z","timestamp":1699888244000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2312995120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,13]]},"references-count":15,"journal-issue":{"issue":"47","published-print":{"date-parts":[[2023,11,21]]}},"alternative-id":["10.1073\/pnas.2312995120"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2312995120","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.07.28.551018","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,13]]},"assertion":[{"value":"2023-07-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2312995120"}}