{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T23:00:30Z","timestamp":1791673230333,"version":"4.3.4"},"reference-count":80,"publisher":"National Academy of Sciences","issue":"14","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["396975076"],"award-info":[{"award-number":["396975076"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006285","name":"Magnus Bergvalls Stiftelse","doi-asserted-by":"publisher","award":["2021-04200"],"award-info":[{"award-number":["2021-04200"]}],"id":[{"id":"10.13039\/501100006285","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2019-04004"],"award-info":[{"award-number":["2019-04004"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["21 1865 Pj"],"award-info":[{"award-number":["21 1865 Pj"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004063","name":"Knut och Alice Wallenbergs Stiftelse","doi-asserted-by":"publisher","award":["2017.009"],"award-info":[{"award-number":["2017.009"]}],"id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,4,2]]},"abstract":"<jats:p>A major consequence of aging and stress, in yeast to humans, is an increased accumulation of protein aggregates at distinct sites within the cells. Using genetic screens, immunoelectron microscopy, and three-dimensional modeling in our efforts to elucidate the importance of aggregate annexation, we found that most aggregates in yeast accumulate near the surface of mitochondria. Further, we show that virus-like particles (VLPs), which are part of the retrotransposition cycle of Ty elements, are markedly enriched in these sites of protein aggregation. RNA interference-mediated silencing of Ty expression perturbed aggregate sequestration to mitochondria, reduced overall protein aggregation, mitigated toxicity of a Huntington\u2019s disease model, and expanded the replicative lifespan of yeast in a partially Hsp104-dependent manner. The results are in line with recent data demonstrating that VLPs might act as aging factors in mammals, including humans, and extend these findings by linking VLPs to a toxic accumulation of protein aggregates and raising the possibility that they might negatively influence neurological disease progression.<\/jats:p>","DOI":"10.1073\/pnas.2313538121","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T15:01:15Z","timestamp":1711378875000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":11,"title":["Elimination of virus-like particles reduces protein aggregation and extends replicative lifespan in\n                    <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6870-2939","authenticated-orcid":false,"given":"K. L.","family":"Schneider","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health\u2014AgeCap, University of Gothenburg, Gothenburg 40530, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health\u2014AgeCap, University of Gothenburg, Gothenburg 40530, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0570-8596","authenticated-orcid":false,"given":"K. S.","family":"Keuenhof","sequence":"additional","affiliation":[{"name":"Department for Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 41390, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. L.","family":"Berglund","sequence":"additional","affiliation":[{"name":"Department for Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 41390, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6804-6564","authenticated-orcid":false,"given":"A.","family":"Fischbach","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health\u2014AgeCap, University of Gothenburg, Gothenburg 40530, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6704-1865","authenticated-orcid":false,"given":"D.","family":"Ahmadpour","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health\u2014AgeCap, University of Gothenburg, Gothenburg 40530, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4083-6459","authenticated-orcid":false,"given":"S.","family":"Chawla","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health\u2014AgeCap, University of Gothenburg, Gothenburg 40530, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8597-3401","authenticated-orcid":false,"given":"P.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health\u2014AgeCap, University of Gothenburg, Gothenburg 40530, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2162-3816","authenticated-orcid":false,"given":"J. L.","family":"H\u00f6\u00f6g","sequence":"additional","affiliation":[{"name":"Department for Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 41390, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3645-0009","authenticated-orcid":false,"given":"P. O.","family":"Widlund","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health\u2014AgeCap, University of Gothenburg, Gothenburg 40530, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5489-2903","authenticated-orcid":false,"given":"T.","family":"Nystr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health\u2014AgeCap, University of Gothenburg, Gothenburg 40530, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,3,25]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.3233\/JPD-201981"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-020-00376-6"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.05.039"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.026"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.07.053"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature21695"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07195"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.08.024"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201106037"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e12-03-0194"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201489524"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-023-01128-6"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2018.00249"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021107913"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717978115"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.258325"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004539"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/2159256X.2016.1154637"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13100-020-00215-x"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MDNA3-0053-2014"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.12.017"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81223-4"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.439307"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201611197"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.111.001933"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008951"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102476"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065810"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2016.04.014"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.24.16802"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.111.12.1717"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201607019"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201403129"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.019208"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204686200"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90108-4"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00331610"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00251-09"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.06.016"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2264806"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/164.3.867"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00684-08"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.119.302376"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1082320"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200807043"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37706-3"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0061(20000630)16:9<785::AID-YEA550>3.0.CO;2-L"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.12.031"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176945"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12047"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100621"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002591"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.233221.113"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3368"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6011"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2015.07.004"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.109.106229"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080418"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06197"},{"key":"e_1_3_4_60_2","first-page":"37","article-title":"Variation in number and volume of the mitochondria in yeast according to growth conditions. A study based on serial sectioning and computer graphics reconstruction","volume":"28","author":"Stevens J. B.","year":"1977","unstructured":"J. B. Stevens, Variation in number and volume of the mitochondria in yeast according to growth conditions. A study based on serial sectioning and computer graphics reconstruction. Biol. Cell. 28, 37\u201356 (1977).","journal-title":"Biol. Cell."},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2300475120"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00806.x"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90110-9"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/318583a0"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.8.12.5245-5256.1988"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.9.4233-4240.2002"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.226.4670.53"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050170042"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.11.5629"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2303358120"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02592.x"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.3389\/fviro.2022.826393"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.2.638-644.2001"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067902"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-92249-1"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1142"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1661-1_4"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1996.0013"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.117.040220"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01242-w"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2313538121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T15:01:47Z","timestamp":1711378907000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2313538121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,25]]},"references-count":80,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2024,4,2]]}},"alternative-id":["10.1073\/pnas.2313538121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2313538121","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,25]]},"assertion":[{"value":"2023-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2313538121"}}