{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T17:02:05Z","timestamp":1781110925653,"version":"3.54.1"},"reference-count":51,"publisher":"National Academy of Sciences","issue":"9","license":[{"start":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T00:00:00Z","timestamp":1708646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"The Ministry of Education, Culture, Sports, Sciences and Technology","doi-asserted-by":"crossref","award":["#19H03705 #22H03123"],"award-info":[{"award-number":["#19H03705 #22H03123"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"The Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["#19K16704 #21K07085"],"award-info":[{"award-number":["#19K16704 #21K07085"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,2,27]]},"abstract":"<jats:p>\n                    Invariant natural killer T (iNKT) cells are innate-like T lymphocytes that express an invariant T cell receptor \u03b1 chain and contribute to bridging innate and acquired immunity with rapid production of large amounts of cytokines after stimulation. Among effecter subsets of iNKT cells, follicular helper NKT (NKT\n                    <jats:sub>FH<\/jats:sub>\n                    ) cells are specialized to help B cells. However, the mechanisms of NKT\n                    <jats:sub>FH<\/jats:sub>\n                    cell differentiation remain to be elucidated. In this report, we studied the mechanism of NKT\n                    <jats:sub>FH<\/jats:sub>\n                    cell differentiation induced by pneumococcal surface protein A and \u03b1-galactosylceramide (P\/A) vaccination. We found that Gr-1\n                    <jats:sup>+<\/jats:sup>\n                    cells helped iNKT cell proliferation and NKT\n                    <jats:sub>FH<\/jats:sub>\n                    cell differentiation in the spleen by producing interleukin-27 (IL-27) in the early phase after vaccination. The neutralization of IL-27 impaired NKT\n                    <jats:sub>FH<\/jats:sub>\n                    cell differentiation, which resulted in compromised antibody production and diminished protection against\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    infection by the P\/A vaccine. Our data indicated that Gr-1\n                    <jats:sup>+<\/jats:sup>\n                    cell\u2013derived IL-27 stimulated mitochondrial metabolism, meeting the energic demand required for iNKT cells to differentiate into NKT\n                    <jats:sub>FH<\/jats:sub>\n                    cells. Interestingly, Gr-1\n                    <jats:sup>+<\/jats:sup>\n                    cell\u2013derived IL-27 was induced by iNKT cells via interferon-\u03b3 production. Collectively, our findings suggest that optimizing the metabolism of iNKT cells was essential for acquiring specific effector functions, and they provide beneficial knowledge on iNKT cell\u2013mediated vaccination-mediated therapeutic strategies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2313964121","type":"journal-article","created":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T13:53:37Z","timestamp":1708696417000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":8,"title":["IL-27 regulates the differentiation of follicular helper NKT cells via metabolic adaptation of mitochondria"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3192-050X","authenticated-orcid":false,"given":"Yasuhiro","family":"Kamii","sequence":"first","affiliation":[{"name":"Department of Bacteriology, The Jikei University School of Medicine, Tokyo 105-8461, Japan"},{"name":"Division of Respiratory Diseases, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Hayashizaki","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, The Jikei University School of Medicine, Tokyo 105-8461, Japan"},{"name":"Jikei Center for Biofilm Science and Technology, The Jikei University School of Medicine, Tokyo 105-8461, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Kanno","sequence":"additional","affiliation":[{"name":"Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Chiba 292-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Chiba","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, The Jikei University School of Medicine, Tokyo 105-8461, Japan"},{"name":"Jikei Center for Biofilm Science and Technology, The Jikei University School of Medicine, Tokyo 105-8461, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taku","family":"Ikegami","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, The Jikei University School of Medicine, Tokyo 105-8461, Japan"},{"name":"Department of Orthopaedic Surgery, The Jikei University School of Medicine, Tokyo 105-8461, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1653-4309","authenticated-orcid":false,"given":"Mitsuru","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, The Jikei University School of Medicine, Tokyo 105-8461, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiro","family":"Akeda","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8088-5165","authenticated-orcid":false,"given":"Toshiaki","family":"Ohteki","sequence":"additional","affiliation":[{"name":"Department of Biodefense Research, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Kubo","sequence":"additional","affiliation":[{"name":"Division of Molecular Pathology, Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba 278-0022, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3108-7383","authenticated-orcid":false,"given":"Kiyotsugu","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, The Jikei University School of Medicine, Tokyo 105-8461, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyoshi","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Oishi","sequence":"additional","affiliation":[{"name":"Toyama Institute of Health, Toyama 939-0363, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Araya","sequence":"additional","affiliation":[{"name":"Division of Respiratory Diseases, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyoshi","family":"Kuwano","sequence":"additional","affiliation":[{"name":"Division of Respiratory Diseases, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 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Kamii IL-27 regulates the differentiation of follicular helper NKT cells via metabolic adaptation of mitochondria. Gene Expression Omnibus at NCBI. https:\/\/www.ncbi.nlm.nih.gov\/geo\/. 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