{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T01:20:55Z","timestamp":1789003255115,"version":"build-2803163510"},"reference-count":38,"publisher":"National Academy of Sciences","issue":"3","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM131715"],"award-info":[{"award-number":["R35GM131715"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01135GM135290"],"award-info":[{"award-number":["R01135GM135290"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["Discovery Grant 2020-04241"],"award-info":[{"award-number":["Discovery Grant 2020-04241"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,1,16]]},"abstract":"<jats:p>Lipid droplets (LDs) are organelles critical for energy storage and membrane lipid homeostasis, whose number and size are carefully regulated in response to cellular conditions. The molecular mechanisms underlying lipid droplet biogenesis and degradation, however, are not well understood. The Troyer syndrome protein spartin (SPG20) supports LD delivery to autophagosomes for turnover via lipophagy. Here, we characterize spartin as a lipid transfer protein whose transfer ability is required for LD degradation. Spartin copurifies with phospholipids and neutral lipids from cells and transfers phospholipids in vitro via its senescence domain. A senescence domain truncation that impairs lipid transfer in vitro also impairs LD turnover in cells while not affecting spartin association with either LDs or autophagosomes, supporting that spartin\u2019s lipid transfer ability is physiologically relevant. Our data indicate a role for spartin-mediated lipid transfer in LD turnover.<\/jats:p>","DOI":"10.1073\/pnas.2314093121","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T15:17:28Z","timestamp":1704727048000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":25,"title":["Spartin-mediated lipid transfer facilitates lipid droplet turnover"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6094-2305","authenticated-orcid":false,"given":"Neng","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8513-3288","authenticated-orcid":false,"given":"Zhouping","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6270-559X","authenticated-orcid":false,"given":"Matthew A. H.","family":"Parson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W2Y2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin L.","family":"Korfhage","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7904-9859","authenticated-orcid":false,"given":"John E.","family":"Burke","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W2Y2, Canada"},{"name":"Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5798-4624","authenticated-orcid":false,"given":"Thomas J.","family":"Melia","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9140-6150","authenticated-orcid":false,"given":"Karin M.","family":"Reinisch","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0085-z"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2023.102178"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202207022"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201807019"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e11-09-0785"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200808041"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-023-01178-w"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng937"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81404-X"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5b06852"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202012058"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0071-5"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13269"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201797359"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-017-0104-3"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom8030045"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38725-w"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj8754"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20220111"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20220206"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702824200"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.259402"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2011442117"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07976"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201811139"},{"key":"e_1_3_4_27_2","article-title":"A fluorescence-based assay of phospholipid scramblase activity","volume":"54635","author":"Ploier B.","year":"2016","unstructured":"B. Ploier, A. K. Menon, A fluorescence-based assay of phospholipid scramblase activity. J. Vis. Exp., 54635 (2016), 10.3791\/54635.","journal-title":"J. Vis. Exp."},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1994.1112"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c01446"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1197-5_22"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.0c00283"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4256"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-0912-y"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0459-y"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1038"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.143"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201905162"},{"key":"e_1_3_4_38_2","unstructured":"M. A. H. Parson J. E. Burke HDX-MS Analysis of CeSpartin and CtSpartinL. PRIDE PRoteomics IDEntifications (PRIDE) Archive. http:\/\/www.ebi.ac.uk\/pride\/archive\/projects\/PXD043175. Deposited 21 June 2023."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2314093121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T15:20:22Z","timestamp":1704727222000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2314093121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,8]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,1,16]]}},"alternative-id":["10.1073\/pnas.2314093121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2314093121","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.11.29.569220","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,8]]},"assertion":[{"value":"2023-08-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2314093121"}}