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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,6,18]]},"abstract":"<jats:p>\n                    Pyruvate lies at a pivotal node of carbon metabolism in eukaryotes. It is involved in diverse metabolic pathways in multiple organelles, and its interorganelle shuttling is crucial for cell fitness. Many apicomplexan parasites harbor a unique organelle called the apicoplast that houses metabolic pathways like fatty acid and isoprenoid precursor biosyntheses, requiring pyruvate as a substrate. However, how pyruvate is supplied in the apicoplast remains enigmatic. Here, deploying the zoonotic parasite\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    as a model apicomplexan, we identified two proteins residing in the apicoplast membranes that together constitute a functional apicoplast pyruvate carrier (APC) to mediate the import of cytosolic pyruvate. Depletion of APC results in reduced activities of metabolic pathways in the apicoplast and impaired integrity of this organelle, leading to parasite growth arrest. APC is a pyruvate transporter in diverse apicomplexan parasites, suggesting a common strategy for pyruvate acquisition by the apicoplast in these clinically relevant intracellular pathogens.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2314314121","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T12:46:56Z","timestamp":1718196416000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":16,"title":["A pyruvate transporter in the apicoplast of apicomplexan parasites"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3449-3876","authenticated-orcid":false,"given":"Pu","family":"Chen","sequence":"first","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei Province, People\u2019s Republic of China"},{"name":"Hubei Hongshan Laboratory, Wuhan 430070, 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Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, Guangdong Province, People\u2019s Republic of China"},{"name":"Frontiers Science Center for Animal Breeding and Sustainable Production, Wuhan 430070, Hubei Province, People\u2019s Republic of 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