{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T02:45:47Z","timestamp":1793155547518,"version":"4.5.0"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"25","license":[{"start":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T00:00:00Z","timestamp":1718236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-17-CE15-0021"],"award-info":[{"award-number":["ANR-17-CE15-0021"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-INBS-04"],"award-info":[{"award-number":["ANR-10-INBS-04"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,6,18]]},"abstract":"<jats:p>\n                    Intracellular bacterial pathogens divert multiple cellular pathways to establish their niche and persist inside their host.\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    , the causative agent of Q fever, secretes bacterial effector proteins via its Type 4 secretion system to generate a\n                    <jats:italic>Coxiella<\/jats:italic>\n                    -containing vacuole (CCV). Manipulation of lipid and protein trafficking by these effectors is essential for bacterial replication and virulence. Here, we have characterized the lipid composition of CCVs and found that the effector Vice interacts with phosphoinositides and membranes enriched in phosphatidylserine and lysobisphosphatidic acid. Remarkably, eukaryotic cells ectopically expressing Vice present compartments that resemble early CCVs in both morphology and composition. We found that the biogenesis of these compartments relies on the double function of Vice. The effector protein initially localizes at the plasma membrane of eukaryotic cells where it triggers the internalization of large vacuoles by macropinocytosis. Then, Vice stabilizes these compartments by perturbing the ESCRT machinery. Collectively, our results reveal that Vice is an essential\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    effector protein capable of hijacking two major cellular pathways to shape the bacterial replicative niche.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2315481121","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T12:50:46Z","timestamp":1718283046000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":13,"title":["The multifunction\n                    <i>Coxiella<\/i>\n                    effector Vice stimulates macropinocytosis and interferes with the ESCRT machinery"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6341-2278","authenticated-orcid":false,"given":"Arthur","family":"Bienvenu","sequence":"first","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Burette","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6634-8331","authenticated-orcid":false,"given":"Franck","family":"Cantet","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon","family":"Gourdelier","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitendriya","family":"Swain","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Cazevieille","sequence":"additional","affiliation":[{"name":"Institut des Neurosciences de Montpellier (INM), Universit\u00e9 de Montpellier, INSERM, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Clemente","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5125-0555","authenticated-orcid":false,"given":"Arif","family":"Sadi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8492-4985","authenticated-orcid":false,"given":"Claire","family":"Dupont","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3241-6834","authenticated-orcid":false,"given":"Manon","family":"Le Fe","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Bonetto","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4977-3939","authenticated-orcid":false,"given":"Benoit","family":"Bordignon","sequence":"additional","affiliation":[{"name":"Montpellier Rio Imaging (MRI), BioCampus Montpellier, CNRS, INSERM, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8517-9342","authenticated-orcid":false,"given":"Delphine","family":"Muriaux","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0835-057X","authenticated-orcid":false,"given":"Stacey","family":"Gilk","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5499-8759","authenticated-orcid":false,"given":"Matteo","family":"Bonazzi","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8568-3725","authenticated-orcid":false,"given":"Eric","family":"Martinez","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS, Universit\u00e9 de Montpellier, Montpellier 34090, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,6,13]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"425","article-title":"Tiny architects: Biogenesis of intracellular replicative niches by bacterial pathogens","volume":"42","author":"Martinez E.","year":"2018","unstructured":"E. Martinez, F. A. Siadous, M. Bonazzi, Tiny architects: Biogenesis of intracellular replicative niches by bacterial pathogens. Fems Microbiol. Rev. 42, 425\u2013447 (2018).","journal-title":"Fems Microbiol. Rev."},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3049"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2016-0044"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1522811113"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00025"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3379"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00490-x"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409716102"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20070670"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.474239"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200804131"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1098188"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2007.00613.x"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092425"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-030212-183750"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1006847"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1215278110"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00696-23"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02484-21"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004013"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12301-7"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200908086"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.123"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003261"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20180019"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00506-2"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0366-5_2"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12715"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M809277200"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201899753"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6646"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247136"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003734"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01765-18"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010136"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02961-22"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2023.102330"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002056"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12066"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148032"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13305"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.12.2505"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2315481121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T12:51:45Z","timestamp":1718283105000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2315481121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,13]]},"references-count":42,"journal-issue":{"issue":"25","published-print":{"date-parts":[[2024,6,18]]}},"alternative-id":["10.1073\/pnas.2315481121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2315481121","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,13]]},"assertion":[{"value":"2023-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2315481121"}}