{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:37:59Z","timestamp":1791362279427,"version":"4.3.1"},"reference-count":67,"publisher":"National Academy of Sciences","issue":"26","license":[{"start":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:00:00Z","timestamp":1718841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Institute of Health","award":["U12AB123456"],"award-info":[{"award-number":["U12AB123456"]}]},{"DOI":"10.13039\/100015911","name":"Joint Genome Institute","doi-asserted-by":"publisher","award":["503546"],"award-info":[{"award-number":["503546"]}],"id":[{"id":"10.13039\/100015911","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015911","name":"Joint Genome Institute","doi-asserted-by":"publisher","award":["DE-AC02-05CH11231"],"award-info":[{"award-number":["DE-AC02-05CH11231"]}],"id":[{"id":"10.13039\/100015911","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019109","name":"DOE | SC | PNNL | Environmental Molecular Sciences Laboratory","doi-asserted-by":"publisher","award":["DE-AC05-76RL01830"],"award-info":[{"award-number":["DE-AC05-76RL01830"]}],"id":[{"id":"10.13039\/100019109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,6,25]]},"abstract":"<jats:p>\n                    Nitrous oxide is a potent greenhouse gas whose production is catalyzed by nitric oxide reductase (NOR) members of the heme-copper oxidoreductase (HCO) enzyme superfamily. We identified several previously uncharacterized HCO families, four of which (eNOR, sNOR, gNOR, and nNOR) appear to perform NO reduction. These families have novel active-site structures and several have conserved proton channels, suggesting that they might be able to couple NO reduction to energy conservation. We isolated and biochemically characterized a member of the eNOR family from the bacterium\n                    <jats:italic>Rhodothermus marinus<\/jats:italic>\n                    and found that it performs NO reduction. These recently identified NORs exhibited broad phylogenetic and environmental distributions, greatly expanding the diversity of microbes in nature capable of NO reduction. Phylogenetic analyses further demonstrated that NORs evolved multiple times independently from oxygen reductases, supporting the view that complete denitrification evolved after aerobic respiration.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2316422121","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T13:40:46Z","timestamp":1718890846000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":36,"title":["Diversity and evolution of nitric oxide reduction in bacteria and archaea"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4073-9910","authenticated-orcid":false,"given":"Ranjani","family":"Murali","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Illinois, Urbana-Champaign, Urbana, IL 61801"},{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125"},{"name":"School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV 89154"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Pace","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Illinois, Urbana-Champaign, Urbana, IL 61801"},{"name":"meliora.bio, Salt Lake City, UT 84103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9607-9897","authenticated-orcid":false,"given":"Robert A.","family":"Sanford","sequence":"additional","affiliation":[{"name":"Department of Earth Science and Environmental Change, University of Illinois at Urbana-Champaign, Urbana, IL 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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