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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,2,27]]},"abstract":"<jats:p>\n                    Cell\u2013cell apical junctions of epithelia consist of multiprotein complexes that organize as belts regulating cell\u2013cell adhesion, permeability, and mechanical tension: the tight junction (\n                    <jats:italic>zonula occludens<\/jats:italic>\n                    ), the\n                    <jats:italic>zonula adherens<\/jats:italic>\n                    (\n                    <jats:italic>ZA<\/jats:italic>\n                    ), and the\n                    <jats:italic>macula adherens<\/jats:italic>\n                    . The prevailing dogma is that at the\n                    <jats:italic>ZA<\/jats:italic>\n                    , E-cadherin and catenins are lined with F-actin bundles that support and transmit mechanical tension between cells. Using super-resolution microscopy on human intestinal biopsies and Caco-2 cells, we show that two distinct multiprotein belts are basal of the tight junctions as the intestinal epithelia mature. The most apical is populated with nectins\/afadin and lined with F-actin; the second is populated with E-cad\/catenins. We name this dual-belt architecture the\n                    <jats:italic>zonula adherens matura<\/jats:italic>\n                    . We find that the apical contraction apparatus and the dual-belt organization rely on afadin expression. Our study provides a revised description of epithelial cell\u2013cell junctions and identifies a module regulating the mechanics of epithelia.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2316722121","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T13:55:21Z","timestamp":1708437321000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":43,"title":["The\n                    <i>zonula adherens matura<\/i>\n                    redefines the apical junction of intestinal epithelia"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8305-7322","authenticated-orcid":false,"given":"Pierre","family":"Mangeol","sequence":"first","affiliation":[{"name":"Aix Marseille Universit\u00e9, CNRS, Institut de Biologie du D\u00e9veloppement de Marseille, IBDM\u2013UMR7288, Marseille 13009, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7727-6898","authenticated-orcid":false,"given":"Dominique","family":"Massey-Harroche","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, CNRS, Institut de Biologie du D\u00e9veloppement de Marseille, IBDM\u2013UMR7288, Marseille 13009, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2468-7006","authenticated-orcid":false,"given":"Michael","family":"Sebbagh","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, INSERM, Dynamics and Nanoenvironment of Biological Membrane, DyNaMo, Turing Center for Living Systems, Marseille 13009, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2748-8797","authenticated-orcid":false,"given":"Fabrice","family":"Richard","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, CNRS, Institut de Biologie du D\u00e9veloppement de Marseille, IBDM\u2013UMR7288, Marseille 13009, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6738-1376","authenticated-orcid":false,"given":"Andr\u00e9","family":"Le Bivic","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, CNRS, Institut de Biologie du D\u00e9veloppement de Marseille, IBDM\u2013UMR7288, Marseille 13009, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1066-7506","authenticated-orcid":false,"given":"Pierre-Fran\u00e7ois","family":"Lenne","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, CNRS, Institut de Biologie du D\u00e9veloppement de Marseille, IBDM\u2013UMR7288, Turing Center for Living Systems, Marseille 13009, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,2,20]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.2.375"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.4.5.529"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90456-0"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90339-7"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.100.1.327"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03563.x"},{"key":"e_1_3_4_7_2","article-title":"al(E)-Catenin is an actin-binding and -bundling protein mediating the attachment of F-actin to the membrane adhesion complex","volume":"5","author":"Rimm D. 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