{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:16:01Z","timestamp":1792451761448,"version":"4.5.0"},"reference-count":61,"publisher":"National Academy of Sciences","issue":"51","license":[{"start":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T00:00:00Z","timestamp":1702252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK060027"],"award-info":[{"award-number":["DK060027"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007753"],"award-info":[{"award-number":["T32GM007753"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM144273"],"award-info":[{"award-number":["T32GM144273"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2023,12,19]]},"abstract":"<jats:p>\n                    The perinatal period is a critical time window in establishing T cell tolerance. Regulatory T cells (Tregs) made during the first 2 wk of life are key drivers of perinatal tolerance induction, but how these cells are generated and operate has not been established. To elucidate the unique environment murine perinatal Tregs encounter within the lymph nodes (LNs) as they first emerge from the thymus, and how it evolves over the succeeding days, we employed single-cell RNA sequencing to generate an atlas of the early LN niche. A highly dynamic picture emerged, the stromal cell compartment showing the most striking changes and putative interactions with other LN cell compartments. In particular, LN stromal cells showed increasing potential for lymphocyte interactions with age. Analogous studies on mice lacking \u03b1:\u03b2 T cells or enriched for autoreactive \u03b1:\u03b2 T cells revealed an acute stromal cell response to \u03b1:\u03b2 T cell dysfunction, largely reflecting dysregulation of Tregs. Punctual ablation of perinatal Tregs induced stromal cell activation that was dependent on both interferon-gamma signaling and activation of conventional CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells. These findings elucidate some of the earliest cellular and molecular events in perinatal induction of T cell tolerance, providing a framework for future explorations.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2316957120","type":"journal-article","created":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T15:11:26Z","timestamp":1702307486000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":4,"title":["Lymph node stromal cell responses to perinatal T cell waves, a temporal atlas"],"prefix":"10.1073","volume":"120","author":[{"given":"Teshika","family":"Jayewickreme","sequence":"first","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Benoist","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Mathis","sequence":"additional","affiliation":[{"name":"Department of Immunology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2023,12,11]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-101320-022432"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3667"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1817"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-00454-2"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0635-3"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006908"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.129.3364.1649"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5256.1728"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199504150-00002"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.4.1613"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5256.1723"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5256.1726"},{"key":"e_1_3_4_13_2","first-page":"209","article-title":"Autoimmune ovarian disease in day 3-thymectomized mice: The neonatal time window, antigen specificity of disease suppression, and genetic control","volume":"293","author":"Tung K. S.","year":"2005","unstructured":"K. S. Tung , Autoimmune ovarian disease in day 3-thymectomized mice: The neonatal time window, antigen specificity of disease suppression, and genetic control. Curr Top. Microbiol. Immunol. 293, 209\u2013247 (2005).","journal-title":"Curr Top. Microbiol. Immunol."},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1075958"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090300"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa7017"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8356452"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8356451"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90068-3"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90262-D"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90748-4"},{"key":"e_1_3_4_22_2","first-page":"293","article-title":"Relative to adult marrow, fetal liver repopulates nearly five times more effectively long-term than short-term","volume":"25","author":"Harrison D. E.","year":"1997","unstructured":"D. E. Harrison , Relative to adult marrow, fetal liver repopulates nearly five times more effectively long-term than short-term. Exp. Hematol. 25, 293\u2013297 (1997).","journal-title":"Exp. Hematol."},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407417101"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay3224"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06423-7"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34868-4"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20182002"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.ade9240"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21246-9"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.04.006"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501191"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2021.06.006"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3920"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3671"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2018.08.003"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri797"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00508-3"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201343759"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature22360"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90246-1"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12419"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/360225a0"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.08.040190.002225"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2006.00426.x"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.156.7.2391"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abb7066"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1800618115"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.08.023"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201242391"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.18.2412"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8171322"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050693"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1428"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21730"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2112"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0591-3"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-00836-7"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3004"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2000748"},{"key":"e_1_3_4_61_2","doi-asserted-by":"crossref","unstructured":"T. Jayewickreme C. Benoist D. Mathis Lymph Node Stromal Cell Responses to Perinatal T Cell Waves A Temporal Atlas. NCBI\u2019s Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE244743. Deposited 5 October 2023.","DOI":"10.1073\/pnas.2316957120"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2316957120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T16:45:56Z","timestamp":1703695556000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2316957120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,11]]},"references-count":61,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2023,12,19]]}},"alternative-id":["10.1073\/pnas.2316957120"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2316957120","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,11]]},"assertion":[{"value":"2023-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2316957120"}}