{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:26:34Z","timestamp":1784078794989,"version":"3.55.0"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"45","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-22-CE15-0041-01"],"award-info":[{"award-number":["ANR-22-CE15-0041-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University for Advanced Studies","award":["USIAS-2020-049"],"award-info":[{"award-number":["USIAS-2020-049"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,11,5]]},"abstract":"<jats:p>\n                    <jats:italic>Shigella<\/jats:italic>\n                    spp. are the causative agents of shigellosis, which remains a leading cause of death in children under the age of 5. Symptoms of shigellosis include bloody diarrhea, associated to colon hemorrhage; in more severe cases\n                    <jats:italic>, Shigella<\/jats:italic>\n                    bacteremia is induced. These clinical features indicate that\n                    <jats:italic>Shigella<\/jats:italic>\n                    are exposed and survive exposure to plasma, locally and systemically, although this has not yet been studied at a molecular level. In this report, we confirmed in a guinea pig model of shigellosis that both\n                    <jats:italic>S. flexneri<\/jats:italic>\n                    5a and\n                    <jats:italic>S. sonnei<\/jats:italic>\n                    induced local hemorrhages and we demonstrated that\n                    <jats:italic>Shigella<\/jats:italic>\n                    reached CD31+\/CD34+ blood vessels located in the mucosa during the late stages of infection, and further disseminated in the bloodstream. These results confirmed the exposure of\n                    <jats:italic>Shigella<\/jats:italic>\n                    to plasma components during its virulence cycle. We demonstrated that all the tested\n                    <jats:italic>Shigella<\/jats:italic>\n                    strains survived plasma exposure in vitro, and we showed that Serine Protease Autotransporters of Enterobacteriaceae (SPATEs) contribute to\n                    <jats:italic>Shigella<\/jats:italic>\n                    dissemination within the colonic mucosa and in the bloodstream. We have confirmed that SPATEs are expressed and secreted in poorly oxygenated environments encountered by\n                    <jats:italic>Shigella<\/jats:italic>\n                    during late infection stages. We further demonstrated that SPATEs promoted\n                    <jats:italic>Shigella<\/jats:italic>\n                    survival in plasma, by cleaving complement component 3 (C3), thereby impairing the complement system activation. We have shown here that the ability of\n                    <jats:italic>Shigella<\/jats:italic>\n                    to survive plasma exposure is a key factor in its virulence, both within primary foci and systemically.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2319951121","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T14:02:39Z","timestamp":1730296959000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":8,"title":["SPATEs promote the survival of\n                    <i>Shigella<\/i>\n                    to the plasma complement system upon local hemorrhage and bacteremia"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2043-8707","authenticated-orcid":false,"given":"Lorine","family":"Debande","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5915-4720","authenticated-orcid":false,"given":"Ahmad","family":"Sabbah","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauriane","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Plateforme prot\u00e9omique Strasbourg Esplanade FRC1589 du CNRS, Universit\u00e9 de Strasbourg, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4847-9375","authenticated-orcid":false,"given":"Richard Patryk","family":"Ngondo","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2866-1199","authenticated-orcid":false,"given":"Julie","family":"Maucotel","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Valente-Barroso","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7015-4791","authenticated-orcid":false,"given":"Antonin C.","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9007-7014","authenticated-orcid":false,"given":"B\u00e9atrice","family":"Roche","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9001-6599","authenticated-orcid":false,"given":"Matthieu","family":"Laborde","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria-Victoria","family":"Cantalapiedra-Mateo","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5980-6859","authenticated-orcid":false,"given":"Tamou","family":"Thahouly","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4530-6350","authenticated-orcid":false,"given":"Ana","family":"Milinski","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), Illkirch 67404, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8498-3694","authenticated-orcid":false,"given":"Laurent","family":"Bianchetti","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), Illkirch 67404, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8883-0874","authenticated-orcid":false,"given":"Christine","family":"Allmang","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut de Biologie Mol\u00e9culaire et Cellulaire (IBMC), Architecture et R\u00e9activit\u00e9 de l\u2019ARN, Strasbourg 67000, 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Debande Data from \u201cSPATEs promote the survival of Shigella to the plasma complement system upon local hemorrhage and bacteremia\u201d. PRIDE. https:\/\/www.ebi.ac.uk\/pride\/archive\/projects\/PXD048033. 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