{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:09:17Z","timestamp":1793210957378,"version":"4.5.0"},"reference-count":67,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2127732"],"award-info":[{"award-number":["2127732"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000015","name":"U.S. Department of Energy","doi-asserted-by":"publisher","award":["DE-AR0001337"],"award-info":[{"award-number":["DE-AR0001337"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,8,6]]},"abstract":"<jats:p>\n                    Many bacteria secrete metallophores, low-molecular-weight organic compounds that bind ions with high selectivity and affinity, in order to access essential metals from the environment. Previous work has elucidated the structures and biosynthetic machinery of metallophores specific for iron, zinc, nickel, molybdenum, and copper. No physiologically relevant lanthanide-binding metallophore has been discovered despite the knowledge that lanthanide metals (Ln) have been revealed to be essential cofactors for certain alcohol dehydrogenases across a diverse range of phyla. Here, we report the biosynthetic machinery, the structure, and the physiological relevance of a lanthanophore, methylolanthanin. The structure of methylolanthanin exhibits a unique 4-hydroxybenzoate moiety which has not previously been described in other metallophores. We find that production of methylolanthanin is required for normal levels of Ln accumulation in the methylotrophic bacterium\n                    <jats:italic>Methylobacterium extorquens<\/jats:italic>\n                    AM1, while overexpression of the molecule greatly increases bioaccumulation and adsorption. Our results provide a clearer understanding of how Ln-utilizing bacteria sense, scavenge, and store Ln; essential processes in the environment where Ln are poorly bioavailable. More broadly, the identification of this lanthanophore opens doors for study of how biosynthetic gene clusters are repurposed for additional functions and the complex relationship between metal homeostasis and fitness.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2322096121","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T11:41:27Z","timestamp":1722339687000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["Identification and characterization of a small-molecule metallophore involved in lanthanide metabolism"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7016-6274","authenticated-orcid":false,"given":"Alexa M.","family":"Zytnick","sequence":"first","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 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Netherlands"},{"name":"Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA 93117"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manh Tri","family":"Phi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Munich 81377, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9490-5913","authenticated-orcid":false,"given":"Nathan M.","family":"Good","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Petras","sequence":"additional","affiliation":[{"name":"Interfaculty Institute of Microbiology and Medicine, Universit\u00e4t T\u00fcbingen, T\u00fcbingen 72074, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2197-136X","authenticated-orcid":false,"given":"Lena J.","family":"Daumann","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Munich 81377, Germany"},{"name":"Chair of Bioinorganic Chemistry, Heinrich-Heine-Universit\u00e4t D\u00fcsseldorf, D\u00fcsseldorf 40225, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4071-7204","authenticated-orcid":false,"given":"Norma Cecilia","family":"Martinez-Gomez","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 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