{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:34:24Z","timestamp":1791315264364,"version":"4.3.1"},"reference-count":28,"publisher":"National Academy of Sciences","issue":"11","license":[{"start":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T00:00:00Z","timestamp":1709510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["NS089534"],"award-info":[{"award-number":["NS089534"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["BX002495"],"award-info":[{"award-number":["BX002495"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["Senior Research Career Scientist Award"],"award-info":[{"award-number":["Senior Research Career Scientist Award"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,3,12]]},"abstract":"<jats:p>The endothelial lining of cerebral microvessels is damaged relatively early after cerebral ischemia\/reperfusion (I\/R) injury and mediates blood\u2013brain barrier (BBB) disruption, neurovascular injury, and long-term neurological deficits. I\/R induces BBB leakage within 1 h due to subtle structural alterations in endothelial cells (ECs), including reorganization of the actin cytoskeleton and subcellular redistribution of junctional proteins. Herein, we show that the protein peroxiredoxin-4 (Prx4) is an endogenous protectant against endothelial dysfunction and BBB damage in a murine I\/R model. We observed a transient upregulation of Prx4 in brain ECs 6 h after I\/R in wild-type (WT) mice, whereas tamoxifen-induced, selective knockout of Prx4 from endothelial cells (eKO) mice dramatically raised vulnerability to I\/R. Specifically, eKO mice displayed more BBB damage than WT mice within 1 to 24 h after I\/R and worse long-term neurological deficits and focal brain atrophy by 35 d. Conversely, endothelium-targeted transgenic (eTG) mice overexpressing Prx4 were resistant to I\/R-induced early BBB damage and had better long-term functional outcomes. As demonstrated in cultures of human brain endothelial cells and in animal models of I\/R, Prx4 suppresses actin polymerization and stress fiber formation in brain ECs, at least in part by inhibiting phosphorylation\/activation of myosin light chain. The latter cascade prevents redistribution of junctional proteins and BBB leakage under conditions of Prx4 repletion. Prx4 also tempers microvascular inflammation and infiltration of destructive neutrophils and proinflammatory macrophages into the brain parenchyma after I\/R. Thus, the evidence supports an indispensable role for endothelial Prx4 in safeguarding the BBB and promoting functional recovery after I\/R brain injury.<\/jats:p>","DOI":"10.1073\/pnas.2400272121","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T15:12:57Z","timestamp":1709565177000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":27,"title":["Endothelial peroxiredoxin-4 is indispensable for blood\u2013brain barrier integrity and long-term functional recovery after ischemic stroke"],"prefix":"10.1073","volume":"121","author":[{"given":"Na","family":"Xu","sequence":"first","affiliation":[{"name":"Pittsburgh Institute of Brain Disorders &amp; Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Pittsburgh Institute of Brain Disorders &amp; Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213"},{"name":"Geriatric Research, Education and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2277-4686","authenticated-orcid":false,"given":"Wenting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Pittsburgh Institute of Brain Disorders &amp; Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213"},{"name":"Geriatric Research, Education and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7502-9201","authenticated-orcid":false,"given":"Yejie","family":"Shi","sequence":"additional","affiliation":[{"name":"Pittsburgh Institute of Brain Disorders &amp; Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213"},{"name":"Geriatric Research, Education and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2817-7417","authenticated-orcid":false,"given":"Rehana K.","family":"Leak","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical Sciences, School of Pharmacy, Duquesne University, Pittsburgh, PA 15282"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard F.","family":"Keep","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, University of Michigan, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ye","sequence":"additional","affiliation":[{"name":"Pittsburgh Institute of Brain Disorders &amp; Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213"},{"name":"Geriatric Research, Education and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuo","family":"Yang","sequence":"additional","affiliation":[{"name":"Pittsburgh Institute of Brain Disorders &amp; Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213"},{"name":"Geriatric Research, Education and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sicheng","family":"Li","sequence":"additional","affiliation":[{"name":"Pittsburgh Institute of Brain Disorders &amp; Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5857-6243","authenticated-orcid":false,"given":"Xiaoming","family":"Hu","sequence":"additional","affiliation":[{"name":"Pittsburgh Institute of Brain Disorders &amp; Recovery and Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213"},{"name":"Geriatric Research, Education and Clinical Center, Veterans Affairs Pittsburgh Health Care System, Pittsburgh, PA 15261"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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