{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:55:10Z","timestamp":1790380510086,"version":"4.1.0"},"reference-count":53,"publisher":"National Academy of Sciences","issue":"36","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["No. 270790 and INV-037720"],"award-info":[{"award-number":["No. 270790 and INV-037720"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100023581","name":"NSF | National Science Foundation Graduate Research Fellowship Program","doi-asserted-by":"publisher","award":["DGE 1745303"],"award-info":[{"award-number":["DGE 1745303"]}],"id":[{"id":"10.13039\/100023581","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV-048977 and OPP1189208"],"award-info":[{"award-number":["INV-048977 and OPP1189208"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,9,3]]},"abstract":"<jats:p>\n                    Elevated bacterial sialidase activity in the female genital tract is strongly associated with poor health outcomes including preterm birth and bacterial vaginosis (BV). These negative effects may arise from sialidase-mediated degradation of the protective mucus layer in the cervicovaginal environment. Prior biochemical studies of vaginal bacterial sialidases have focused solely on the BV-associated organism\n                    <jats:italic>Gardnerella vaginalis<\/jats:italic>\n                    . Despite their implications for sexual and reproductive health, sialidases from other vaginal bacteria have not been characterized. Here, we show that vaginal\n                    <jats:italic>Prevotella<\/jats:italic>\n                    species produce sialidases that possess variable activity toward mucin substrates. The sequences of sialidase genes and their presence are largely conserved across clades of\n                    <jats:italic>Prevotella<\/jats:italic>\n                    from different geographies, hinting at their importance globally. Finally, we find that\n                    <jats:italic>Prevotella<\/jats:italic>\n                    sialidase genes and transcripts, including those encoding mucin-degrading sialidases from\n                    <jats:italic>Prevotella timonensis<\/jats:italic>\n                    , are highly prevalent and abundant in human vaginal genomes and transcriptomes. Together, our results identify\n                    <jats:italic>Prevotella<\/jats:italic>\n                    as a critical source of sialidases in the vaginal microbiome, improving our understanding of this detrimental bacterial activity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2400341121","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T15:05:06Z","timestamp":1724684706000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["<i>Prevotella<\/i>\n                    are major contributors of sialidases in the human vaginal microbiome"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1508-2034","authenticated-orcid":false,"given":"Paula","family":"Pelayo","sequence":"first","affiliation":[{"name":"Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima A.","family":"Hussain","sequence":"additional","affiliation":[{"name":"Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard, Massachusetts General Hospital, Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline A.","family":"Werlang","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe M.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin M.","family":"Woolston","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138"},{"name":"Department of Chemical Engineering, Northeastern University, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0499-1895","authenticated-orcid":false,"given":"Claire M.","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay","family":"Rutt","sequence":"additional","affiliation":[{"name":"Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"France","sequence":"additional","affiliation":[{"name":"Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 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