{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T21:05:09Z","timestamp":1793394309030,"version":"4.5.0"},"reference-count":51,"publisher":"National Academy of Sciences","issue":"19","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100022963","name":"Key Research and Development Program of Zhejiang Province","doi-asserted-by":"publisher","award":["2021C03100"],"award-info":[{"award-number":["2021C03100"]}],"id":[{"id":"10.13039\/100022963","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Zhejiang Provincial Natural Science Foundation","doi-asserted-by":"crossref","award":["LQ21C120001 LZ21H040001"],"award-info":[{"award-number":["LQ21C120001 LZ21H040001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070829 32100676 32370900"],"award-info":[{"award-number":["32070829 32100676 32370900"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,5,7]]},"abstract":"<jats:p>\n                    In the meiotic prophase, programmed DNA double-strand breaks are repaired by meiotic recombination. Recombination-defective meiocytes are eliminated to preserve genome integrity in gametes. BRCA1 is a critical protein in somatic homologous recombination, but studies have suggested that BRCA1 is dispensable for meiotic recombination. Here we show that BRCA1 is essential for meiotic recombination. Interestingly, BRCA1 also has a function in eliminating recombination-defective oocytes.\n                    <jats:italic>Brca1<\/jats:italic>\n                    knockout (KO) rescues the survival of\n                    <jats:italic>Dmc1<\/jats:italic>\n                    KO oocytes far more efficiently than removing CHK2, a vital component of the DNA damage checkpoint in oocytes. Mechanistically, BRCA1 activates chromosome asynapsis checkpoint by promoting ATR activity at unsynapsed chromosome axes in\n                    <jats:italic>Dmc1<\/jats:italic>\n                    KO oocytes. Moreover,\n                    <jats:italic>Brca1<\/jats:italic>\n                    KO also rescues the survival of asynaptic\n                    <jats:italic>Spo11<\/jats:italic>\n                    KO oocytes. Collectively, our study not only unveils an unappreciated role of chromosome asynapsis in eliminating recombination-defective oocytes but also reveals the dual functions of BRCA1 in safeguarding oocyte genome integrity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2401386121","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T13:38:59Z","timestamp":1714657139000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":22,"title":["BRCA1 safeguards genome integrity by activating chromosome asynapsis checkpoint to eliminate recombination-defective oocytes"],"prefix":"10.1073","volume":"121","author":[{"given":"Long","family":"Bai","sequence":"first","affiliation":[{"name":"Key Laboratory of Reproductive Genetics (Ministry of Education) and Women\u2019s Reproductive Health Laboratory of Zhejiang Province, Women\u2019s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, 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