{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:49:04Z","timestamp":1789069744111,"version":"build-2803163510"},"reference-count":62,"publisher":"National Academy of Sciences","issue":"37","license":[{"start":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T00:00:00Z","timestamp":1725321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32030013"],"award-info":[{"award-number":["32030013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31821001"],"award-info":[{"award-number":["31821001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,9,10]]},"abstract":"<jats:p>\n                    Ovarian development was traditionally recognized as a \u201cdefault\u201d sexual outcome and therefore received much less scientific attention than testis development. In turtles with temperature-dependent sex determination (TSD), how the female pathway is initiated to induce ovary development remains unknown. In this study, we have found that phosphorylation of the signal transducer and activator of transcription 3 (pSTAT3) and\n                    <jats:italic>Foxl2<\/jats:italic>\n                    exhibit temperature-dependent sexually dimorphic patterns and tempo-spatial coexpression in early embryos of the red-eared slider turtle (\n                    <jats:italic>Trachemys scripta elegans<\/jats:italic>\n                    ). Inhibition of pSTAT3 at a female-producing temperature of 31 \u00b0C induces 64.7% female-to-male sex reversal, whereas activation of pSTAT3 at a male-producing temperature of 26 \u00b0C triggers 75.6% male-to-female sex reversal. In addition, pSTAT3 directly binds to the locus of the female sex-determining gene\n                    <jats:italic>Foxl2<\/jats:italic>\n                    and promotes\n                    <jats:italic>Foxl2<\/jats:italic>\n                    transcription. Overexpression or knockdown of\n                    <jats:italic>Foxl2<\/jats:italic>\n                    can rescue the sex reversal induced by inhibition or activation of pSTAT3. This study has established a direct genetic link between warm temperature-induced STAT3 phosphorylation and female pathway initiation in a TSD system, highlighting the critical role of pSTAT3 in the cross talk between female and male pathways.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2401752121","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T13:50:19Z","timestamp":1725371419000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":23,"title":["pSTAT3 activation of\n                    <i>Foxl2<\/i>\n                    initiates the female pathway underlying temperature-dependent sex determination"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3399-1205","authenticated-orcid":false,"given":"Pengfei","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People\u2019s Republic of China"},{"name":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4310-609X","authenticated-orcid":false,"given":"Xifeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chutian","family":"Ge","sequence":"additional","affiliation":[{"name":"Institute of Animal Sex and Development, Zhejiang Wanli University, Ningbo 315100, People\u2019s Republic of China"},{"name":"College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Animal Sex and Development, Zhejiang Wanli University, Ningbo 315100, People\u2019s Republic of China"},{"name":"College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People\u2019s Republic of China"},{"name":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1868-5664","authenticated-orcid":false,"given":"Weiguo","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,9,3]]},"reference":[{"key":"e_1_3_4_1_2","volume-title":"Temperature-Dependent Sex Determination in Vertebrates","author":"Valenzuela N.","year":"2004","unstructured":"N. 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