{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:01:43Z","timestamp":1789012903969,"version":"build-2803163510"},"reference-count":59,"publisher":"National Academy of Sciences","issue":"47","license":[{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI165575"],"award-info":[{"award-number":["R01AI165575"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"HHS | NIH | National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01DC007864"],"award-info":[{"award-number":["R01DC007864"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014543","name":"UC | UCSB | Institute for Collaborative Biotechnologies","doi-asserted-by":"publisher","award":["W911NF-19-2-0026"],"award-info":[{"award-number":["W911NF-19-2-0026"]}],"id":[{"id":"10.13039\/100014543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,11,19]]},"abstract":"<jats:p>\n                    Attraction and mating between male and female animals depend on effective communication between conspecifics. However, in mosquitoes, we have only a rudimentary understanding of the sensory cues and receptors critical for the communication that is essential for reproductive behavior. While it is known that male\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    use sound to help them identify females, it is not unclear whether sound detection is absolutely required since other cues such as vision may also participate in mating behavior. To determine the effect of eliminating hearing on mating success, we knocked out the\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    TRPVa channel, which is a protein expressed in chordotonal neurons in the Johnston\u2019s organ (JO) that respond to sound-induced movements in the antenna. Loss of\n                    <jats:italic>trpVa<\/jats:italic>\n                    eradicated sound-induced responses from the JO, thereby abolishing hearing. Strikingly, mutation of\n                    <jats:italic>trpVa<\/jats:italic>\n                    eliminated mating behavior in males. In contrast,\n                    <jats:italic>trpVa<\/jats:italic>\n                    -null females mated, although this behavior was slightly delayed relative to wild-type females. Males and females produce sounds as they beat their wings at distinct frequencies during flight. Sound mimicking the female wingbeat induced flight, attraction, and copulatory-like behavior in wild-type males without females present, but not in\n                    <jats:italic>trpVa<\/jats:italic>\n                    -null males. Males are known to modulate their wingbeat frequencies before mating in the air, which is a phenomenon referred to as rapid frequency modulation (RFM). We found that RFM was absent in mosquitoes lacking TRPVa. We conclude that the requirement for\n                    <jats:italic>trpVa<\/jats:italic>\n                    and hearing for male reproductive behavior in\n                    <jats:italic>Aedes<\/jats:italic>\n                    is absolute, as mating in the deaf males is eliminated.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2404324121","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T15:05:48Z","timestamp":1730732748000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":10,"title":["Deafness due to loss of a TRPV channel eliminates mating behavior in\n                    <i>Aedes aegypti<\/i>\n                    males"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8002-2766","authenticated-orcid":false,"given":"Yijin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1797-7163","authenticated-orcid":false,"given":"Dhananjay","family":"Thakur","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Duge","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Girling","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas A.","family":"DeBeaubien","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8603-4046","authenticated-orcid":false,"given":"Benjamin H.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adishthi S.","family":"Gurav","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5637-1482","authenticated-orcid":false,"given":"Craig","family":"Montell","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, CA 93106"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,11,4]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/35098592"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.119.302263"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8209251"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2660(08)00603-2"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01065443"},{"key":"e_1_3_4_6_2","volume-title":"The Mosquitoes of North and Central America and the West Indies. 1. A General Consideration of Mosquitoes, Their Habits, and Their Relations to the Human Species","author":"Howard L. O.","year":"1912","unstructured":"L. O. Howard, H. G. Dyar, F. Knab, The Mosquitoes of North and Central America and the West Indies. 1. A General Consideration of Mosquitoes, Their Habits, and Their Relations to the Human Species (Carnegie Institution of Washington, Washington, D.C., 1912)."},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.2307\/2421604"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2007.10.007"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01939928"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2012.07.003"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1410488111"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.43963"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2020.11.009"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2021.715759"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.08347"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-050715-105122"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2019.01.001"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.101.2622.335"},{"key":"e_1_3_4_20_2","first-page":"97","article-title":"Auditory apparatus of the Culex mosquito","volume":"3","author":"Johnston C.","year":"1855","unstructured":"C. Johnston, Auditory apparatus of the Culex mosquito. Q. J. Microsc. Sci. 3, 97\u2013102 (1855).","journal-title":"Q. J. Microsc. Sci."},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1166541"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-38599-3"},{"key":"e_1_3_4_23_2","first-page":"2039","article-title":"A role for acoustic distortion in novel rapid frequency modulation behaviour in free-flying male mosquitoes","volume":"219","author":"Sim\u00f5es P. M.","year":"2016","unstructured":"P. M. Sim\u00f5es, R. A. Ingham, G. Gibson, I. J. Russell, A role for acoustic distortion in novel rapid frequency modulation behaviour in free-flying male mosquitoes. J. Exp. Biol. 219, 2039\u20132047 (2016).","journal-title":"J. Exp. 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K.","year":"1971","unstructured":"W. K. Hartberg, Observations on mating behavior of Aedes aegypti in nature. Bull. World Health Organ. 45, 000\u2013847 (1971).","journal-title":"Bull. 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Thakur C. Montell Data from \u201cDeafness eliminates mating behavior in Aedes aegypti males\u201d. Dryad. https:\/\/doi.org\/10.5061\/dryad.qz612jmrb. Deposited 11 October 2016."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2404324121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T15:05:58Z","timestamp":1730732758000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2404324121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,4]]},"references-count":59,"journal-issue":{"issue":"47","published-print":{"date-parts":[[2024,11,19]]}},"alternative-id":["10.1073\/pnas.2404324121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2404324121","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,4]]},"assertion":[{"value":"2024-02-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2404324121"}}