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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,6,11]]},"abstract":"<jats:p>\n                    Transcription is extremely important for cellular processes but can be hindered by RNA polymerase II (RNAPII) pausing and stalling. Cockayne syndrome protein B (CSB) promotes the progression of paused RNAPII or initiates transcription-coupled nucleotide excision repair (TC-NER) to remove stalled RNAPII. However, the specific mechanism by which CSB initiates TC-NER upon damage remains unclear. In this study, we identified the indispensable role of the ARK2N\u2013CK2 complex in the CSB-mediated initiation of TC-NER. The ARK2N\u2013CK2 complex is recruited to damage sites through CSB and then phosphorylates CSB. Phosphorylation of CSB enhances its binding to stalled RNAPII, prolonging the association of CSB with chromatin and promoting CSA-mediated ubiquitination of stalled RNAPII. Consistent with this finding,\n                    <jats:italic>\n                      Ark2n\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice exhibit a phenotype resembling Cockayne syndrome. These findings shed light on the pivotal role of the ARK2N\u2013CK2 complex in governing the fate of RNAPII through CSB, bridging a critical gap necessary for initiating TC-NER.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2404383121","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T13:41:55Z","timestamp":1717681315000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":13,"title":["The ARK2N\u2013CK2 complex initiates transcription-coupled repair through enhancing the interaction of CSB with lesion-stalled RNAPII"],"prefix":"10.1073","volume":"121","author":[{"given":"Yefei","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Radiation Medicine, School of Basic Medical Sciences, Peking University International Cancer Institute, Institute of Advanced Clinical Medicine, State Key Laboratory of Molecular Oncology, Peking University Health Science Center, Beijing 100191, 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