{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:25:28Z","timestamp":1790677528069,"version":"4.1.0"},"reference-count":52,"publisher":"National Academy of Sciences","issue":"24","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,11,21]]},"abstract":"<jats:p>\n                    The target of rapamycin protein (TOR) is a highly conserved\n ataxia telangiectasia-related protein kinase essential for cell growth.\n Emerging evidence indicates that TOR signaling is highly complex and is\n involved in a variety of cellular processes. To understand its general\n functions, we took a chemical genomics approach to explore the genetic\n interaction between TOR and other yeast genes on a genomic scale. In\n this study, the rapamycin sensitivity of individual deletion mutants\n generated by the\n                    <jats:italic>Saccharomyces<\/jats:italic>\n                    Genome Deletion Project\n was systematically measured. Our results provide a global view of the\n rapamycin-sensitive functions of TOR. In contrast to conventional\n genetic analysis, this approach offers a simple and thorough analysis\n of genetic interaction on a genomic scale and measures genetic\n interaction at different possible levels. It can be used to study the\n functions of other drug targets and to identify novel protein\n components of a conserved core biological process such as DNA damage\n checkpoint\/repair that is interfered with by a cell-permeable\n chemical compound.\n                  <\/jats:p>","DOI":"10.1073\/pnas.240444197","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:41:48Z","timestamp":1027680108000},"page":"13227-13232","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":136,"title":["A chemical genomics approach toward understanding the global functions of the target of rapamycin protein (TOR)"],"prefix":"10.1073","volume":"97","author":[{"given":"Ting-Fung","family":"Chan","sequence":"first","affiliation":[{"name":"Departments of Pathology and Immunology and\r Genetics, Washington University School of Medicine, St.\r Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Immunology and\r Genetics, Washington University School of Medicine, St.\r Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Riles","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Immunology and\r Genetics, Washington University School of Medicine, St.\r Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. F. Steven","family":"Zheng","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Immunology and\r Genetics, Washington University School of Medicine, St.\r Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2000,11,14]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(99)80007-0"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(99)80070-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1715094"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90144-F"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90141-4"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.5.1.105"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)31592-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90058-6"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.46.27531"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.7.1.25"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.7.3963"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.15.1904"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.7.3070"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.8.4463"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.4.987"},{"key":"e_1_3_3_16_2","unstructured":"Bertram P. G. Choi J. Carvalho J. Ai W. D. Zeng C. B. Chan T. F. & Zheng X. F. S. (2000) J. Biol. Chem. in press."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.14866"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/45287"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.24.3271"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19970930)13:12<1099::AID-YEA177>3.0.CO;2-7"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(99)01425-5"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/124.2.251"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90015-5"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5287.546"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/4462"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5442.1153"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/35001009"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5429.901"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.1.69"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(07)00535-0"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008651"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.14.7.4522-4531.1994"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/135.1.17"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00314439"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00330954"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)77262-1"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.0240a.x"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.15.1.1"},{"key":"e_1_3_3_39_2","first-page":"229","volume-title":"The Molecular and Cellular Biology of the Yeast Saccharomyces","author":"Jones E W","year":"1997","unstructured":"E W Jones, E C Webb, D A Shaywitz The Molecular and Cellular Biology of the Yeast Saccharomyces, eds J Pringle, J Broach, E Jones (Cold Spring Harbor Lab. Press, Plainview, NY) 3, 229\u2013470 (1997)."},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.30.1.405"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.425"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.68.1.583"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/372570a0"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00599-6"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004120050335"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/25292"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.13.1.261"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.1.135"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81893-0"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/46558"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1296-450"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/6791"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.240444197","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T19:58:28Z","timestamp":1650916708000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.240444197"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11,14]]},"references-count":52,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2000,11,21]]}},"alternative-id":["10.1073\/pnas.240444197"],"URL":"https:\/\/doi.org\/10.1073\/pnas.240444197","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,11,14]]},"assertion":[{"value":"2000-05-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-09-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-11-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}