{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:51:16Z","timestamp":1789055476780,"version":"build-2803163510"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["J.0225.20; F.4501.15; T.0067.21; 7.4586.19; 7.6529.21; J.0094.15; T.0241.19"],"award-info":[{"award-number":["J.0225.20; F.4501.15; T.0067.21; 7.4586.19; 7.6529.21; J.0094.15; T.0241.19"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022434","name":"Walloon excellence in life sciences and biotechnology","doi-asserted-by":"publisher","award":["A-14 12; A-15"],"award-info":[{"award-number":["A-14 12; A-15"]}],"id":[{"id":"10.13039\/501100022434","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"EC | European Regional Development Fund","doi-asserted-by":"publisher","award":["411132-957270"],"award-info":[{"award-number":["411132-957270"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,8,6]]},"abstract":"<jats:p>\n                    Alcelaphine gammaherpesvirus 1 (AlHV-1) asymptomatically persists in its natural host, the wildebeest. However, cross-species transmission to cattle results in the induction of an acute and lethal peripheral T cell lymphoma-like disease (PTCL), named malignant catarrhal fever (MCF). Our previous findings demonstrated an essential role for viral genome maintenance in infected CD8\n                    <jats:sup>+<\/jats:sup>\n                    T lymphocytes but the exact mechanism(s) leading to lymphoproliferation and MCF remained unknown. To decipher how AlHV-1 dysregulates T lymphocytes, we first examined the global phenotypic changes in circulating CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells after experimental infection of calves. T cell receptor repertoire together with transcriptomics and epigenomics analyses demonstrated an oligoclonal expansion of infected CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells displaying effector and exhaustion gene signatures, including GZMA, GNLY, PD-1, and TOX2 expression. Then, among viral genes expressed in infected CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, we uncovered A10 that encodes a transmembrane signaling protein displaying multiple tyrosine residues, with predicted ITAM and SH3 motifs. Impaired A10 expression did not affect AlHV-1 replication in vitro but rendered AlHV-1 unable to induce MCF. Furthermore, A10 was phosphorylated in T lymphocytes in vitro and affected T cell signaling. Finally, while AlHV-1 mutants expressing mutated forms of A10 devoid of ITAM or SH3 motifs (or both) were able to induce MCF, a recombinant virus expressing a mutated form of A10 unable to phosphorylate its tyrosine residues resulted in the lack of MCF and protected against a wild-type virus challenge. Thus, we could characterize the nature of this \u03b3-herpesvirus-induced PTCL-like disease and identify an essential mechanism explaining its development.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2404536121","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T13:17:56Z","timestamp":1722518276000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":5,"title":["Unraveling clonal CD8 T cell expansion and identification of essential factors in \u03b3-herpesvirus-induced lymphomagenesis"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8328-9804","authenticated-orcid":false,"given":"Meijiao","family":"Gong","sequence":"first","affiliation":[{"name":"Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine\u2014Fundamental and Applied Research for Animals &amp; Health (FARAH), University of Li\u00e8ge, Li\u00e8ge 4000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7147-480X","authenticated-orcid":false,"given":"Fran\u00e7oise","family":"Myster","sequence":"additional","affiliation":[{"name":"Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine\u2014Fundamental and Applied Research for Animals &amp; Health (FARAH), University of Li\u00e8ge, Li\u00e8ge 4000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulkader","family":"Azouz","sequence":"additional","affiliation":[{"name":"Institute for Medical Immunology, Universit\u00e9 Libre de Bruxelles, Gosselies 6041, Belgium"},{"name":"Center for Research in Immunology, Universit\u00e9 Libre de Bruxelles, Gosselies 6041, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillem","family":"Sanchez Sanchez","sequence":"additional","affiliation":[{"name":"Institute for Medical Immunology, Universit\u00e9 Libre de Bruxelles, Gosselies 6041, Belgium"},{"name":"Center for Research in Immunology, Universit\u00e9 Libre de Bruxelles, Gosselies 6041, Belgium"},{"name":"Department of Pharmacotherapy and Pharmaceutics, Universit\u00e9 Libre de Bruxelles, Brussels 1050, Belgium"},{"name":"Walloon Excellence in Life Sciences and Biotechnology (WELBIO), WEL Research Institute, Wavre 1300, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shifang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine\u2014Fundamental and Applied Research for Animals &amp; 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Gong Deep sequencing after alcelaphine gammaherpesvirus 1 infection reveals the nature of CD8+ T cell expansion and identify an essential viral protein for fatal bovine malignant catarrhal fever. Gene Expression Omnibus (GEO). https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE253729. 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