{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T21:39:26Z","timestamp":1793741966269,"version":"4.6.1"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"37","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["5R35GM139430"],"award-info":[{"award-number":["5R35GM139430"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013443","name":"Swedish Institute","doi-asserted-by":"publisher","award":["2023-02312"],"award-info":[{"award-number":["2023-02312"]}],"id":[{"id":"10.13039\/100013443","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,9,10]]},"abstract":"<jats:p>\n                    While the acute inflammatory response to harmful stimuli is protective, unrestrained neutrophil swarming drives collateral tissue damage and inflammation. Biosynthesized from omega-3 essential polyunsaturated fatty acids, resolvins are a family of signaling molecules produced by immune cells within the resolution phase to orchestrate return to homeostasis. Understanding the mechanisms that govern biosynthesis of these potent molecules gives insight into stimulating endogenous resolution and offers fresh opportunities for preventing and treating excessive inflammation. In this report, using materials prepared by total synthesis and liquid chromatography and tandem mass spectrometry-based matching studies, we established the role of 7,8(S,S)-epoxytetraene intermediate in the biosynthesis of resolvin D1, resolvin D2, and the resolvin conjugate in tissue regeneration (RCTR1) by human phagocytes. We demonstrated that this 7,8(S,S)-epoxy-containing intermediate is directly converted to resolvin D2 by human M2-like macrophages and to resolvin D1 and RCTR1 by human macrophages, neutrophils, and peripheral blood mononuclear cells. In addition, both human recombinant soluble epoxide hydrolase (sEH) and the glutathione S-transferase leukotriene C\n                    <jats:sub>4<\/jats:sub>\n                    synthase (LTC\n                    <jats:sub>4<\/jats:sub>\n                    S) each catalyze conversion of this epoxide to resolvin D1 and RCTR1, respectively. MS\n                    <jats:sup>3<\/jats:sup>\n                    ion-trap scans and isotope incorporation of\n                    <jats:sup>18<\/jats:sup>\n                    O from H\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>18<\/jats:sup>\n                    O with sEH indicated that the oxygen atom at C-8 in resolvin D1 is derived from water. Results from molecular docking simulations with biosynthetic precursor 17S-hydroperoxy-4,7,10,13,19-\n                    <jats:italic>cis<\/jats:italic>\n                    -15-\n                    <jats:italic>trans<\/jats:italic>\n                    -docosahexaenoic acid and the epoxy intermediate were consistent with 5-lipoxygenase production of resolvin D1. Together, these results give direct evidence for the role of resolvin 7,8(S,S)-epoxytetraene intermediate in the endogenous formation of resolution-phase mediators resolvin D1, resolvin D2, and RCTR1 by human phagocytes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2405821121","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T14:32:13Z","timestamp":1725546733000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["Biosynthesis of resolvin D1, resolvin D2, and RCTR1 from 7,8(S,S)-epoxytetraene in human neutrophils and macrophages"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0832-9938","authenticated-orcid":false,"given":"Robert","family":"Nshimiyimana","sequence":"first","affiliation":[{"name":"Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6945-9619","authenticated-orcid":false,"given":"M\u00e9lissa","family":"Simard","sequence":"additional","affiliation":[{"name":"Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8489-3955","authenticated-orcid":false,"given":"Tarvi","family":"Teder","sequence":"additional","affiliation":[{"name":"Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm S-171 77, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4802-2845","authenticated-orcid":false,"given":"Ana R.","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Neuroscience, Virtua Health College of Medicine &amp; Life Sciences of Rowan University, Stratford, NJ 08084"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7581-4741","authenticated-orcid":false,"given":"Bernd W.","family":"Spur","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Neuroscience, Virtua Health College of Medicine &amp; Life Sciences of Rowan University, Stratford, NJ 08084"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1823-5153","authenticated-orcid":false,"given":"Jesper Z.","family":"Haeggstr\u00f6m","sequence":"additional","affiliation":[{"name":"Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm S-171 77, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4627-8545","authenticated-orcid":false,"given":"Charles N.","family":"Serhan","sequence":"additional","affiliation":[{"name":"Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.03.016"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020760"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13479"},{"key":"e_1_3_4_4_2","volume-title":"Cells, Tissues, and Disease: Principles of General Pathology","author":"Majno G.","year":"2004","unstructured":"G. Majno, I. Joris, Cells, Tissues, and Disease: Principles of General Pathology (Oxford University Press, New York, ed. 2, 2004).","edition":"2"},{"key":"e_1_3_4_5_2","first-page":"155","volume-title":"Advances in Prostaglandin, Thromboxane, and Leukotriene Research","author":"Marfat A.","year":"1985","unstructured":"A. Marfat, E. J. Corey, \u201cSynthesis and structure elucidation of leukotrienes\u201d in Advances in Prostaglandin, Thromboxane, and Leukotriene Research, J. E. Pike, D. R. Morton Jr., Eds. (Raven Press, New York, 1985), pp. 155\u2013228."},{"key":"e_1_3_4_6_2","first-page":"45","volume-title":"Advances in Prostaglandin, Thromboxane, and Leukotriene Research","author":"Samuelsson B.","year":"1985","unstructured":"B. Samuelsson, S. Hammarstr\u00f6m, M. Hamberg, C. N. Serhan, \u201cStructural determination of leukotrienes and lipoxins\u201d in Advances in Prostaglandin, Thromboxane, and Leukotriene Research, J. E. Pike, D. R. Morton Jr., Eds. (Raven Press, New York, 1985), pp. 45\u201371."},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.X112.351437"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2116559118"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1039\/D2RA01537D"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M609212200"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-051921-084047"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.32647"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2017.08.007"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.2147\/NDT.S417630"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2023.117446"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apha.2022.12.005"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.123.032588"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2018.01.004"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyx023"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvae039"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.121.000712"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2017.03.041"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.26932"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-051921-085014"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300218200"},{"key":"e_1_3_4_26_2","first-page":"151","volume-title":"Advances in Prostaglandin, Thromboxane, and Leukotriene Research","author":"Egan R. W.","year":"1983","unstructured":"R. W. Egan , \u201cSpecific inhibition and oxidative regulation of 5-lipoxygenase\u201d in Advances in Prostaglandin, Thromboxane, and Leukotriene Research, B. Samuelsson, R. Paoletti, P. W. Ramwell, Eds. (Raven Press, New York, 1983), pp. 151\u2013157."},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(88)90233-0"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08541"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.3.689"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)85161-8"},{"key":"e_1_3_4_31_2","first-page":"3654","article-title":"Measurement and characterization of membrane-bound and soluble epoxide hydrolase activities in resting mononuclear leukocytes from human blood","volume":"44","author":"Seidegard J.","year":"1984","unstructured":"J. Seidegard , Measurement and characterization of membrane-bound and soluble epoxide hydrolase activities in resting mononuclear leukocytes from human blood. Cancer Res. 44, 3654\u20133660 (1984).","journal-title":"Cancer Res."},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/bph.16009"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpclett.0c02052"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI142883"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20150225"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902003R"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.176047"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202001952R"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.584206"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25042309"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41366-024-01482-x"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28124728"},{"key":"e_1_3_4_43_2","doi-asserted-by":"crossref","unstructured":"R. Nshimiyimana C. N. Serhan Raw dataset from \u201cBiosynthesis of resolvin D1 resolvin D2 and RCTR1 from 7 8(S S)-epoxytetraene in human neutrophils and macrophages\u201d. BioStudies. https:\/\/www.ebi.ac.uk\/biostudies\/studies\/S-BSST1612. Deposited 21 August 2024.","DOI":"10.1073\/pnas.2405821121"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2405821121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T15:38:51Z","timestamp":1732721931000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2405821121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,5]]},"references-count":43,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2024,9,10]]}},"alternative-id":["10.1073\/pnas.2405821121"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2405821121","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,5]]},"assertion":[{"value":"2024-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2405821121"}}