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U.S.A."],"published-print":{"date-parts":[[2024,12,10]]},"abstract":"<jats:p>\n                    Cortical condensates, transient punctate-like structures rich in actin and the actin nucleation pathway member Neural Wiskott-Aldrich syndrome protein (N-WASP), form during activation of the actin cortex in the\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    oocyte. Their emergence and spontaneous dissolution is linked to a phase separation process driven by chemical kinetics. However, the mechanisms that drive the onset of cortical condensate formation near membranes remain unexplored. Here, using a reconstituted phase separation assay of cortical condensate proteins, we demonstrate that the key component, N-WASP, can collectively undergo surface condensation on supported lipid bilayers via a prewetting transition. Actin partitions into the condensates, where it polymerizes and counteracts the N-WASP prewetting transition. Taken together, the dynamics of condensate-assisted cortex formation appear to be controlled by a balance between surface-assisted condensate formation and polymer-driven condensate dissolution. This opens perspectives for understanding how the formation of complex intracellular structures is affected and controlled by phase separation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2407497121","type":"journal-article","created":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T13:47:11Z","timestamp":1733320031000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":15,"title":["Actin polymerization counteracts prewetting of N-WASP on supported lipid bilayers"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9018-3303","authenticated-orcid":false,"given":"Tina","family":"Wiegand","sequence":"first","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","place":["Dresden, Germany"]},{"name":"Max Planck Institute for the Physics of Complex Systems","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2770-8415","authenticated-orcid":false,"given":"Jinghui","family":"Liu","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","place":["Dresden, Germany"]},{"name":"Center for Systems Biology Dresden","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Vogeley","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"LuValle-Burke","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","place":["Dresden, Germany"]},{"name":"Max Planck Institute for the Physics of Complex Systems","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Geisler","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","place":["Dresden, Germany"]},{"name":"Max Planck School Matter to Life","place":["Heidelberg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anatol W.","family":"Fritsch","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","place":["Dresden, Germany"]},{"name":"Max Planck Institute for the Physics of Complex Systems","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0851-704X","authenticated-orcid":false,"given":"Anthony A.","family":"Hyman","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","place":["Dresden, Germany"]},{"name":"Cluster of Excellence Physics of Life","place":["Dresden, Germany"]},{"name":"Technische Universit\u00e4t Dresden","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2290-5826","authenticated-orcid":false,"given":"Stephan W.","family":"Grill","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics","place":["Dresden, Germany"]},{"name":"Cluster of Excellence Physics of Life","place":["Dresden, Germany"]},{"name":"Technische Universit\u00e4t Dresden","place":["Dresden, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2024,12,4]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.09.016"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad9964"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau5721"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2301881120"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf4382"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.7"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.49.1565"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1088\/0034-4885\/62\/12\/201"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41567-021-01462-2"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2103401118"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1088\/1367-2630\/ac320b"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-022-00882-3"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","unstructured":"D. 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