{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T15:45:47Z","timestamp":1792251947647,"version":"4.4.0"},"reference-count":66,"publisher":"National Academy of Sciences","issue":"44","license":[{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Insitute of Virology and Bacteriology, Programme EXCELES","award":["LX22NPO2103"],"award-info":[{"award-number":["LX22NPO2103"]}]},{"name":"Czech Academy of Sciences","award":["Praemium Academiae Award 2018"],"award-info":[{"award-number":["Praemium Academiae Award 2018"]}]},{"name":"Czech Academy of Sciences","award":["RVO 68378050-KAV-NPUI"],"award-info":[{"award-number":["RVO 68378050-KAV-NPUI"]}]},{"name":"Ministry of Education, Youth, and Sports of the Czech Republic","award":["LM2023052"],"award-info":[{"award-number":["LM2023052"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2024,10,29]]},"abstract":"<jats:p>\n                    Syncytin-1, a human fusogenic protein of retroviral origin, is crucial for placental syncytiotrophoblast formation. To mediate cell-to-cell fusion, Syncytin-1 requires specific interaction with its cognate receptor. Two trimeric transmembrane proteins, Alanine, Serine, Cysteine Transporters 1 and 2 (ASCT1 and ASCT2), were suggested and widely accepted as Syncytin-1 cellular receptors. To quantitatively assess the individual contributions of human ASCT1 and ASCT2 to the fusogenic activity of Syncytin-1, we developed a model system where the\n                    <jats:italic>ASCT1<\/jats:italic>\n                    and\n                    <jats:italic>ASCT2<\/jats:italic>\n                    double knockout was rescued by ectopic expression of either ASCT1 or ASCT2. We demonstrated that ASCT2 was required for Syncytin-1 binding, cellular entry, and cell-to-cell fusion, while ASCT1 was not involved in this receptor interaction. We experimentally validated the ASCT1\u2013ASCT2 heterotrimers as a possible explanation for the previous misidentification of ASCT1 as a receptor for Syncytin-1. This redefinition of receptor specificity is important for proper understanding of Syncytin-1 function in normal and pathological pregnancy.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2407519121","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T15:01:33Z","timestamp":1729522893000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":22,"title":["Receptor usage of Syncytin-1: ASCT2, but not ASCT1, is a functional receptor and effector of cell fusion in the human placenta"],"prefix":"10.1073","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8708-606X","authenticated-orcid":false,"given":"Kry\u0161tof","family":"\u0160tafl","sequence":"first","affiliation":[{"name":"Laboratory of Viral and Cellular Genetics, Institute of Molecular Genetics of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 14220, Czech 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